三七总皂甙对慢性低氧大鼠右心室心肌细胞钙电流的影响  

Effects of panax notoginseng sponins on L- type Ca^(2+) current of the right ventricle myocytes from chronic hypoxic rats

作　　者：朱智勇[1] 王晓睛 杨映宁[1] 周定邦[1] 

机构地区：[1]昆明医学院基础医学院生理学教研室,云南昆明650031

出　　处：《中国病理生理杂志》2004年第3期399-401,共3页Chinese Journal of Pathophysiology

基　　金：云南省自然科学基金(No.1999C0070M)

摘　　要：目的:探讨长期应用三七总皂甙对慢性常压低氧大鼠右心室心肌细胞钙电流的影响。方法:采用全细胞膜片钳方法分别记录并比较正常对照组、慢性低氧组、慢性低氧加三七总皂甙组大鼠单个右心室心肌细胞钙电流和电流-电压关系曲线。结果:正常对照组、慢性低氧组、慢性低氧加三七总皂甙组细胞钙电流峰值分别为(571.1±145.9)pA,(835.1±194.1)pA,(526.3±152.6)pA。缺氧组显著大于另两组(P<0.05)。结论:应用三七总皂甙能阻滞慢性低氧引起的钙内流增高。AIM: To study the effect of panax notoginseng sponins (PNS) on L-type Ca^(2+) current in isolated right ventricle myocytes from chronic hypoxic rats. METHODS: Using whole cell patch clamp recording technique,we measured I_(Ca-L) in isolated right ventricle myocytes which were divided into three group: control group, chronic hypoxic group and chronic hypoxic group with PNS (100 mg·kg^(-1)·d^(-1)). RESULTS: Tne result showed I_(Ca-L) of cells from chronic hypoxic group were significantly larger than the other two groups(P<0.05). CONCLUSION: PNS decreases L-type Ca^(2+) current of the right ventricle myocytes from chronic hypoxic rats.

关 键 词：三七总皂甙 缺氧 大鼠 心肌 钙电流 膜片钳术 

分 类 号：R285.5[医药卫生—中药学] R363[医药卫生—中医学]