昆布多糖硫酸酯抑制BxPC-3细胞增殖的实验研究  

Experimental study of LAMS restrain multiplication of BxPC -3 cells

作　　者：肖青[1] 董蒲江[2] 胡妮妮[1] 戴勤[1] 

机构地区：[1]重庆医科大学附属第一医院血液内科,400016 [2]重庆医科大学附属第一医院器官移植研究室,400016

出　　处：《重庆医学》2004年第3期417-418,共2页Chongqing medicine

摘　　要：目的　探讨LAMS对BxPC 3细胞增殖的抑制作用。方法　以MTT法分别测定不同浓度LAMS对BxPC 3细胞增殖的抑制作用 ,对以LAMS处理前后的BxPC 3细胞分别用TUNEL(POD)法测定凋亡细胞、免疫组化法测定其凋亡 ,Bcl 2、BaX基因蛋白含量。结果　LAMS可使BxPC 3细胞增殖抑制 ,细胞Bcl 2基因蛋白表达下降、BaX基因蛋白表达增加。结论　LAMS可有效地抑制BxPCObjective To study the effects of LAMS for restrain multiplication of BxPC 3 cells. Methods MTT was used to determine the inhibiting action in the different concentration LAMS on BxPC 3 cells. TUNEL(POD) was used to determine the number of opoptosis cells which were treated with or without LAMS and the amount of Bcl 2?BaX was examined by using immunohistochemical staining.Results LAMS can effectively restrain the proliferation and apoptosis of cells on BxPC 3 cells and Bcl 2 expression decrease,but BaX expression increase.Conclusion LAMS can effectively restrain the proliferation on BxPC 3 cells.

关 键 词：昆布多糖硫酸酯 胰腺肿瘤 

分 类 号：R285[医药卫生—中药学]