丝裂霉素C酸活化机理研究  

STUDY ON THE MECHANISM OF ACTIVATION OF MITOMYCIN C

作　　者：丁敏[1] 程圭芳[1] 赵洁[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《理化检验（化学分册）》2004年第3期125-128,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助(29875008)

摘　　要：丝裂霉素C(MMC)是一种广谱抗肿瘤药物,它在体内活化后可以与DNA以共价结合或交联反应等模式相互作用,从而抑制肿瘤细胞DNA的合成。文中首次采用光谱学方法,结合紫外 可见光谱、圆二色光谱手段,提出了MMC的酸活化机理。证明它是一个质子化水解过程,同时推导了酸活化第一步基元反应的反应级数和反应速率常数的计算公式,并计算得该基元反应的速率常数k为3.083×10-4s-1。Mitomycin C (MMC) is a broad-spectrum antitumor drug, which inhibits the synthesis of DNA of the tumour cell, due to the covalent or interlinking interaction with DNA after its activation in human body. The mechanism of the acid activation of MMC is studied for the first time by spectrometry, including the UV-VIS spectrophotometry and the circular dichroism spectrometry. It is proved that it is a process of protonated hydrolysis. The reaction order of first step of the basic reaction of acid activation is deduced and the constant of reaction rate k is calculated to have a value of 3.083×10^(-4)s^(-1).

关 键 词：丝裂霉素C 抗肿瘤药物 酸活化机理 药理学 质子化水解过程 反应级数 反应速率常数 基元反应 光谱学 肿瘤分子DNA 

分 类 号：R979.1[医药卫生—药品]