热休克反应对急性运动大鼠心肌自由基代谢的影响  

Effects of Heat Shock Response on Free Radical Metabolism in Rat Heart after an Acute Exercise

作　　者：李建平[1] 张葆欣[2] 王瑞元[3] 叶春[4] 

机构地区：[1]中央民族大学体育系,北京100081 [2]西安体育学院,陕西西安710068 [3]北京体育大学,北京100084 [4]南京师范大学,江苏南京210029

出　　处：《北京体育大学学报》2004年第2期209-211,共3页Journal of Beijing Sport University

摘　　要：目的:探讨热休克反应对急性运动大鼠心肌自由基代谢的影响。方法:SD大鼠随机分为:室温安静组,室温运动组,热休克反应安静组,热休克反应运动组。热休克反应组进行热暴露处理;热暴露处理24h后,运动组大鼠进行一次急性运动,急性运动结束16h后取样。检测各组心肌组织中MDA含量和SOD的活性,检测安静组心肌组织HSP72的含量。结果:1)与室温安静组相比,热休克安静组大鼠心肌组织中的HSP72浓度显著升高(P<0.01);热休克反应安静组大鼠心肌SOD活性升高、MDA含量降低,与室温安静组相比变化不明显(P>0.05)。2)热休克反应运动组心肌MDA含量显著低于室温运动组(P<0.01),SOD活性高于室温运动组,但无统计学意义(P>0.05)。结论:热休克反应可减轻急性运动所致大鼠心肌组织SOD活性下降的程度,抑止MDA含量的升高。说明热休克反应预处理能够减轻急性运动大鼠心肌的抗氧化能力,减少自由基的生成,减轻自由基所致的损伤,对急性运动所致心肌损伤具有一定的保护作用。This study is designed to test the effect of heat shock response on rat heart after an acute bout exercise. Male rats are randomly assigned to one of the four experimental groups (n=8/group): normothermic sedentary (NS), heat shock response sedentary (HS), normothermic exercise (NE), and heat shock response exercise (HE). Rats in the NS and HS groups are exposed to 15 minutes of whole body hyperthermia (colonic temperature 41℃-41.5℃). Twenty-four hours after the heat shock response, HE and NE groups are subjected to an acute bout exercise. Rat hearts are removed after exercise; the relative levels of heat shock protein 72(HSP72), the activity of SOD and the concentration of MDA are analyzed. The following results are obtained: 1) The relative levels of HSP72 in HS group are significantly higher than those in NS group(P<0.01); the activity of SOD is increased and the concentration of MDA is decreased(P>0.05). 2) Compared with NE group, the activity of SOD in HE group is elevated(P>0.05). The concentration of MDA in HE group is significantly lower than that in NS group(P<0.01). The results suggest that heat shock response can protect against free radical injury in rat hearts deduced by an acute bout exercise.

关 键 词：热休克反应 急性运动 心肌 自由基 

分 类 号：G804.7[文化科学—运动人体科学]