筛选差异表达基因的方法进展  

The Progress in the Methods for Screening Differentially Expressed Genes

作　　者：李文雍[1] 陈清轩[2] 

机构地区：[1]阜阳师范学院生物系,阜阳236032 [2]中科院遗传与发育生物学研究所,北京100080

出　　处：《阜阳师范学院学报（自然科学版）》2004年第1期1-6,共6页Journal of Fuyang Normal University(Natural Science)

基　　金：国家重点基础研究发展规划项目 973基金资助 (G2 0 0 0 0 16 10 7)

摘　　要：植入前胚胎差异表达基因的筛选是分离、鉴定发育相关基因的关键 ,是克隆动物发育分子机理研究的基础 .哺乳动物植入前胚胎和克隆胚胎材料的获得十分不易 ,使得筛选差异表达基因的方法在该领域的应用均受到较大的限制 .以DDRT -PCR为基础的单胚mRNA差异表达技术的建立 ,为克隆植入前胚胎发育相关基因及哺乳动物克隆胚胎发育分子机理研究提供有力的工具 ,本文将在多年研究基础上对该技术及目前筛选差异表达基因的方法进行综述 .Screening of differential display gene in implantation embryos is the key of isolation and identification development-related genes and is the basis of studying developmental molecuar mechanism of cloning animal. It's difficulty to obtain mammal implantation embryos and cloning embryos, which restricts the methods of screening differential display gene. On the basis of DDRT-PCR, we established the single embryo mRNA differential display reverse transcript polymerase chain reaction, which is a very important method of studying the developmental gene of cloning implantation embryos and the molecular mechanism of mammal cloned embryos development. This paper will give an emphases on the single embryo DDRT-PCR and some strategies of screening new genes based on our studies.

关 键 词：差异表达基因 单胚mRNA差异显示 分子生物学技术 基因筛选 克隆 

分 类 号：Q78[生物学—分子生物学]