高效液相色谱法测定血清瓜氨酸与国人正常值  

Determination of serum citrulline levels in normal Chinese people with HPLC

作　　者：刘放南[1] 谭力[1] 罗楠[1] 蒋峰[1] 李元新[1] 李幼生[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2004年第2期116-117,共2页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金资助项目 (批准号 :3 0 2 712 63 )

摘　　要：目的 :用高效液相色谱法检测血清瓜氨酸的正常值。　方法 :抽取 33名正常人空腹血 ,将异硫氰酸苯酯(PITC)作为柱前衍生剂 ,用乙酸钠和甲醇、乙晴做二元梯度洗脱 ,30min完成分离。　结果 :本实验中血清瓜氨酸测定方法具有良好的线性 ,相关系数为 0 .9974。　结论 :高效液相色谱检测血清瓜氨酸稳定可靠 ,PITC作为衍生剂灵敏度高 ,用此方法测出的国人正常值为 (1 6 .87± 5 .97) μmol/L。Objectives: To determine plasma citrulline levels in 33 healthy Chinese by High Performance Liquid Chromatography (HPLC). Methods: PITC was used as pre column derivative of HPLC. Two phases gradient elution was performed with acetic sodium and cyanide for 30 minutes. Results: The linearity of the measurement was fine (with r=0.9974). Conclusions: HPLC is a stable and efficient method for the determination of plasma citrulline. PITC is an ideal derivative with high sensitivity. Plasma citrulline levels in normal Chinese are (16.87±5.97) μmol/L.

关 键 词：高效液相色谱法 血清 瓜氨酸 正常值 非蛋白质氨基酸 色谱条件 

分 类 号：R446.11[医药卫生—诊断学]