低剂量T-2毒素对大鼠血清生化指标的影响  

Effect of minidoses T-2 toxin on biochemical indexes in rat serum

作　　者：姜昕[1] 刘密凤[1] 林林[1] 孙殿军[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病研究所,150086

出　　处：《中国地方病学杂志》2004年第2期116-118,共3页Chinese Jouranl of Endemiology

基　　金：黑龙江省杰出青年课题基金资助项目(J01鄄04)

摘　　要：目的探讨低剂量T鄄2毒素(<100μg/kg)对机体生化指标的影响。方法选用Wistar大鼠30只,在饲料中按10、50、100μg/kg添加T鄄2毒素,5周后,测定血清中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH鄄Px)、腺苷脱氨酶(ADA)、羟脯氨酸(HYP)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、γ鄄谷氨酰转肽酶(γ鄄GT)、肌酸激酶(CK)、α鄄羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH),并加以比较分析。结果除GSH鄄Px、HYP随给毒剂量的增加而下降,γ鄄GT改变不明显外,其余7项指标均随给毒剂量增加而上升。其中有统计学意义的有MDA(P<0.01)、GSH鄄Px(P<0.01)、HYP(P<0.05)、AST(P<0.05)、LDH(P<0.05)。结论低剂量T鄄2毒素可致机体多种生化指标改变,提示低剂量T鄄2毒素可能引起机体多器官损伤。Objective To examine the effect of minidose (< 100 μg/kg) T-2 toxin on rat. Methods Thirty Wistar rats were divided into four groups, the experimental groups were fed fodder with T-2 toxin of 10 μg/kg, 50 μg/kg, and 100 μg/kg, respectively for 5 weeks. Reagent kits and biochemical auto-analysis apparatus were used to detect the biochemical indexes in serum. Results The content of GHS-Px and HYP were reduced with the increase of T-2 toxin, that of γ-GT had no obvious change, and the other indexes increased with the increase of T-2 toxin, in which MDA (P < 0.01), GSH-Px (P < 0.01), HYP(P < 0.05), AST (P < 0.05) and LDH (P < 0.05) had statistic significance. Conclusion T-2 toxin of minidoses can cause many biochemical indexes change, these results show that T-2 toxin of minidoses can cause damage in many organs of rat.

关 键 词：低剂量 T-2毒素 大鼠 血清 生化指标 细胞毒性 

分 类 号：R115[医药卫生—公共卫生与预防医学]