肌红蛋白在硅基表面自组装的研究  

The Self-Assembly Study of Myoglobin on Silicon Surface

作　　者：陈浩[1] 刘小鹏[1] 陈慧兰[1] 

机构地区：[1]南京大学化学系,配位化学国家重点实验室,南京210093

出　　处：《无机化学学报》2004年第4期451-454,共4页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金资助项目(No.50272029);博士点基金资助项目(No.200028401)。

摘　　要：With the rapid development of bioanalytical methods and biosensors,it is desirable to immobilize a Self-as-sembled Monolayers with pure protein on inorganic surface.In this work,we tried to immobilize myoglobin on a surface of a silicon substrate as a model.At first,the cleaned silicon substrate was filled with5%3-aminopropy-ltrimethoxysilane(3-APTS).Then the formed SAMs were treated with glutaraldehyde and solution of myoglobin.Subsequently,the composition,structure and morphology of the thin film surface have been characterized by X-ray photoelectron spectroscopy(XPS)and Atomic force microscopy(AFM).The results indicate that myoglobin had been successfully immobilized on P100Si substrate.This method will be especially useful for further protein biomolecule immobilization and advanced single molecule detection studies.With the rapid development of bioanalytical methods and biosensors,it is desirable to immobilize a Self-as-sembled Monolayers with pure protein on inorganic surface.In this work,we tried to immobilize myoglobin on a surface of a silicon substrate as a model.At first,the cleaned silicon substrate was filled with5%3-aminopropy-ltrimethoxysilane(3-APTS).Then the formed SAMs were treated with glutaraldehyde and solution of myoglobin.Subsequently,the composition,structure and morphology of the thin film surface have been characterized by X-ray photoelectron spectroscopy(XPS)and Atomic force microscopy(AFM).The results indicate that myoglobin had been successfully immobilized on P100Si substrate.This method will be especially useful for further protein biomolecule immobilization and advanced single molecule detection studies.

关 键 词：肌红蛋白 硅基 自组装单层膜 光电子能谱 原子力显微镜 生物传感器 生物芯片 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] Q523[自动化与计算机技术—控制科学与工程]