吴茱萸中吴茱萸碱和吴茱萸次碱的HPLC分析  

Determination of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth by HPLC

作　　者：甄攀[1] 王治宝[1] 白雪梅[1] 张万明[1] 李如章[1] 刘淑芬 

机构地区：[1]张家口医学院,河北张家口075000 [2]昌黎县环保局,河北秦皇岛066000

出　　处：《中成药》2004年第3期227-229,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :建立用高效液相色谱法 (HPLC)测量中药吴茱萸中吴茱萸碱和吴茱萸次碱的含量的方法。方法 :以乙酸乙酯为溶剂用超声震荡提取法提取吴茱萸碱和吴茱萸次碱。提取液用HPLC法分析 ,色谱柱 :YWG C18(10 μm ,2 5 0mm×4 .6mm)。流动相 :水 甲醇 乙腈 (42∶2 0∶38)。流速 1.0ml·min-1。柱温 :30℃。检测波长 :2 6 0nm。结果 :吴茱萸碱的回归方程为Y =5 6 .4 7X +2 9.89,相关系数为 0 .9997,线性范围为 5～ 10 0 0 μg·mL-1,回收率为 99.83% ,日内和日间RSD分别为 0 .85 %和 1.4 0 % ;吴茱萸次碱的回归方程为Y =4 9.0 8X +2 7.10 ,相关系数为 0 .9999,线性范围为 4 .5～ 90 0 μg·mL-1,回收率为 98.72 % ,日内和日间RSD分别为 0 .6 3%和 1.17%。本文所用吴茱萸中吴茱萸碱和吴茱萸次碱的含量分别为 0 .387%和 0 .30 9%。结论 :方法稳定 ,重现性好 ,操作简单 ,耗费时间短。是检测吴茱萸中吴茱萸碱和吴茱萸次碱较理想的方法。AIM: To establish a method for determination of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth by HPLC. METHODS : Evodiamine and rutaecarpine were extracted with the solvent ethyl acetate and the ultrasonic extractor. The separation was performed on YWG-C_ 18 (4.6mm×250mm,10μm) column with water-methanol-acetonitrile (42∶20∶38) as mobile phase. The flow rate was 1.0ml·min -1 , the column temperature was at 30℃ and the wavelength of UV detector was at 260nm. RESULTS : For evodiamine,the regression equation was Y=56.47X+29.89,r =0.9997, the linar range was within 5～1000μg and the recovery rate was 99.83%. the inter-day and intra-day precision (RSD) were 0.85% and 1.40%,respectively. For rutaecarpine,the regression equation was Y=49.08X+27.10,r= 0.9999,the linear range was within 4.5 ～900μg,the recovery rate was 98.72%. the inter-day and intra-day precision (RSD) were 0.63% and 1.17% ,respectively. The contents of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth were 0.387% and 0.309% respectively. CONCLUSION : The method for determination of evodiamine and rutaecarpine by HPLC is simple,accurate,rapid,sensitive and with good reproducibility. It is a better method to determine evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth.

关 键 词：吴茱萸 吴茱萸碱 吴茱萸次碱 HPLC 

分 类 号：R284.1[医药卫生—中药学]