黄花倒水莲总苷预防性给药对高脂血症家兔的调脂作用  

Studies on the Lipid Regulating Effects of Saponins from Polygala Aureocauda

作　　者：李浩[1] 王秋娟[1] 吴锦慧[1] 朱丹妮[2] 

机构地区：[1]中国药科大学生理教研室,南京210009 [2]中国药科大学中药复方研究室,南京210038

出　　处：《中国天然药物》2004年第2期115-118,共4页

基　　金：国家中医药管理局新药基金 (No .DZX0 2 1A)资助项目~~

摘　　要：目的 :利用家兔饵食性高血脂症模型研究黄花倒水莲总苷调脂作用。方法 :连续给予新西兰家兔 30d高脂饲料 ,使形成高脂模型 ,造模同时ig给予黄花倒水莲总苷 (PTS) 2 5 ,5 0 ,10 0mg/kg。停止高脂饲料喂养后继续给药 30d。分别于给药前 ,给药后第 30天和第 6 0天测定血清中TC、TG、HDL C和LDL C。于第 6 0天测定血清中SOD活性、MDA、ApoA1、ApoB含量并取肝组织测定TC、TG含量。结果 :高脂饵食造模后第 30天 ,第6 0天血清中TC ,TG ,和LDL C及MDA ,肝组织中TC ,TG含量升高 ,血清中HDL C含量和SOD活性降低。PTS能降低血清中TC ,TG ,LDL C及MDA ,肝组织中TC、TG ,升高血清中HDL C含量和SOD活性。结论 :高脂饲料喂养新西兰家兔 30d即可形成高脂模型 ;黄花倒水莲总皂苷预防性给药能降低新西兰家兔血清中TC ,TG ,LDL C及MDA含量 ,肝组织中TC ,TG含量并升高血清中HDL C含量和SOD活性并呈剂量相关性。ApoA1随HDL C升高而升高 ,ApoB则随LDL C减少而减少。AIM:The lipid regulating effect of PTS was studied in rabbits fed with high lipid diet.METHOD:the rabbits were fed with high fatty diet for 30 consecutive days as hyperlipidemia model.Meanwhile they were administered with the different concentrations(25,50,100 mg/kg) of PTS. After stopping high fatty diet the rubbits were continued to adminster for 30 d. The concentration of TC (total cholesterol),TG (triglyceride),low-density lipoprotein cholesterol (LDL-C),and high-density lipoprotein cholesterol (HDL-C)in serum was determined before feeding with high fatty diet,and at the 30 th day and the 60 th day after feeding respectively .The concentration of TC,TG in live,ApoA1,ApoB,MDA (malondialdehyde) and activity of SOD were determined at the 60 th day after feeding.RESULT: After 30d and 60d the concentration of TC,TG,LDL-C and MDA in serum,and TC,TG in liver increased,but HDL-C and the activity of SOD in serum decreased. PTS can decrease the serum TC, TG, LDL-C, ApoB and MDA,but can increase the serum HDL-C, ApoA1 and activity of SOD. CONCLUSION: The rabbit hyperlipidemia model was established after feeding with high fatty diet for 30 days; The results suggested that PTS could decrease the concentration of TC,TG, LDL-C,MDA in serum and TC, TG in liver,but increase the concentration of HDL-C and the activity of SOD in serum. All of these have interrelation with the dosage. ApoA1 increases with the HDL-C increase, ApoB decreases with the LDL-C decrease.

关 键 词：预防性给药 高脂血症 家兔 调脂作用 黄花倒水莲总苷 药物治疗 

分 类 号：R259.892[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]