树鼩CXCR4 cDNA的克隆和序列分析  

Cloning and Sequence Analysis of CXCR4 cDNA from Tree Shrew (Tupaia belangeri)

作　　者：杨敏[1] 贲昆龙[1] 

机构地区：[1]中国科学院昆明动物研究所,昆明650223

出　　处：《中国实验动物学报》2004年第1期3-6,共4页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的　获得树CXCR4的cDNA序列 ,探讨其是否可以支持HIV_1病毒和细胞的结合。方法　设计相应的引物 ,用RT_PCR ,基因克隆 ,DNA序列分析技术。结果　获得了全长为 10 59bp树CXCR4(tsCXCR4)基因的cDNA。发现其核苷酸序列与人的CXCR4(hCXCR4)基因的cDNA有 92 8%的相似性 ,由此推导出的氨基酸序列有 96 9%相似性。与hCXCR4功能相关的关键位点完全相同 ,tsCXCR4的N端第 7和 12位点为酪氨酸 ,第 14、15和3 2位点为谷氨酸 ,胞外环第 183 ,188为精氨酸 ,第 193、2 62位点以及跨膜区 97位点为天冬氨酸。结论　树的CXObjectiveTo acquire cDNA sequence of tree shrew CXCR4 and to understand whether tree shrew CXCR4 (tsCXCR4) supports the HIV_1 binding. MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used. ResultsThe clone of full_length tsCXCR4 cDNA is 1059bp. The homology of tsCXCR4 with that of human CXCR4 (hCXCR4) is 92 8% at nucleotide level and 96 9% at deduced amino acid level. Tyr residues are at positions 7 and 12, and Glu residues are at positions 14,15 and 32 in th e NH 2 _terminal extracellular domain of the tsCXCR4. In addition, Arg residues at positions 183 and 188, and Asp residues at positions 193, 262 and 97 are found in the extracellular loops and transmembrane domains of the tsCXCR4, the above residues are the same as hCXCR4, and are essential for the function of hCXCR4. ConclusionThese results suggest that tsCXCR4 may contribute to the entry of X4 and R5X4 strains of HIV_1.

关 键 词：树鼩 CXCR4 CDNA 克隆 序列分析 HIV-1 受体 

分 类 号：Q785[生物学—分子生物学]