非动情期SD大鼠子宫内膜异位症模型的建立  

Establishment of Endometriosis Model in SD Rats during Non-estrus

作　　者：张薇[1] 沈洪沁[2] 黄启玲 洪波[2] 谢玲玲[1] 陈嘉[1] 陈小霞[1] 邹移海[1] 

机构地区：[1]广州中医药大学实验动物中心,广州510405 [2]广州市中医院,广州510130 [3]湖北省钟祥市中医院,431900

出　　处：《中国实验动物学报》2004年第1期21-24,共4页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的　研究SD大鼠非动情期子宫内膜异位症建立方法和异位病灶的组织学形态及体液免疫反应。方法　采用外科诱导法对 40只非动情期和 40只动情期SD雌性大鼠行子宫组织自体移植手术。采集造模前后的血液进行体液免疫IgG、IgA、IgM、C3 、C4 的观察 ;手术后 7周取健侧子宫中段的在位内膜和移植的异位内膜行组织学研究。结果　SD大鼠非动情期进行子宫组织自体异位种植术成模率 95% ,与大鼠动情期进行子宫组织自体异位种植术成模率 94 8%比较 ,二者差异无显著性 ;异位内膜上皮细胞功能活跃 ,有类似正位子宫内膜的周期变化 ,其生理特征与正位子宫内膜基本相同。且造模后大鼠体液免疫反应敏感IgG、C3 增高 (P <0 0 1、3 P <0 0 5) ;IgM降低 (P <0 0 1)、IgA、C4 差异无显著性。ObjectiveTo establish a method of the endomet r iosis in SD rats during non-estrus and study its histological structure and hum oral immunity.Methods\ Endometriosis was induced in 40 female SD rat s during non-estrus and estrus respectively by surgery of self-endometrium tra nsplant.Before and after the operation,its blood was collected for observing IgG ,IgA,IgM,C 3,C 4.The endometrium of the uninjured side and the ectopic endomet rium were taken for histological observation.Results\ There was no s i gnificant difference between the successful rates,95% of non-estrus contrast to 94 8% of estrus by this method.The function of the ectopic endometrium epithel ial cells was activated,with similar altered cycle or physiological characterist ic as their normal endometrium.After operation,IgG and C3 were increased(P< 0 01,P<0 05);IgM reduced(P<0 01) and with no significant change f or IgA, C4.Conclusion\ The endometriosis model of SD rats can be successfully mad e both during non-estrus and estrus by self-endometrium transplant.

关 键 词：非动情期 动情期 子宫内膜异位症 大鼠 体液免疫 

分 类 号：R711.71[医药卫生—妇产科学]