斑点杂交试验直接检测血清HBV DNA的临床意义  

CLINICAL SIGNIFICANCE OF DIRECT SPOT HYBRIDIZATION IN SERUM HBV DNA

作　　者：许永成[1] 黄雅英[1] 余永清 吴成安[1] 钟农英 张晓天[1] 刁联硕 

机构地区：[1]合肥市传染病医院

出　　处：《安徽医学》1989年第4期5-6,共2页Anhui Medical Journal

摘　　要：本文应用斑点杂交试验直接检测101例乙肝病毒(HBV)无症状携带者、慢活肝及肝硬化患者血清HBV DNA,阳性率分别为58.3％(21/36)、36.9％(17/46)、10.5％(2/21),三组间有显著差异(P<0.01)。并与血清免疫标志进行比较,在HBeAg阳性时,HBV DNA检出率分别为95.2％(20/21)、70.8％(17/24)、25.0％(1/4),三组间有显著差异(P<0.05)。HBsAg、抗-HBc及抗-HBe与血清HBV DNA并非一致。结果表明,HBV无症状携带者比慢活肝及肝硬化具有更高的传染性,是极重要的传染源。One hundred and one patients with HBV asymptomatic carrier, chronic active hepatitis (CAN) and liver cirrhosis were assayed for HBV DNA with direct spot hydridization. HBV DNA positive rates were58.3% (21/36), 39.6% (17/46) and 10.5% (2/21), respectively. There was significant difference among three groups (p<0.01). HBV DNA positive rates were 95.2% (20/21), 70.8% (17/24) and 25.0% (1/4) , respectively, when HBeAg was positive. There was significant difference among three groups (p<0.05). It is not accordant that HBsAg, anti-HBc and anti-HBe were compared with serum HBV DNA. These results indicate that HBV asymptomatic carriers are more infectious than CAH and liver cirrhosis. They are extremely infectious sources.

关 键 词：斑点杂交 HBV DNA 血清 

分 类 号：R446.5[医药卫生—诊断学]