拟单性木兰种子DNA提取方法的研究  

Study on Separation Method of DNA from Parakmeria Seed

作　　者：杨萍[1,2] 胡永萍 王正加[1] 

机构地区：[1]浙江林学院生命科学学院 [2]浙江工业大学生物与环境学院,浙江杭州310014 [3]浙江省杭州天目药业研发中心

出　　处：《浙江林业科技》2004年第1期24-26,38,共4页Journal of Zhejiang Forestry Science and Technology

摘　　要：以云南拟单性木兰种子为材料,对三种DNA的提取方法(CTAB法、SDS法、高盐—低pH值法)进行了比较。结果表明:CTAB法最适于拟单性木兰种子DNA的提取,且适宜于RAPD分析,SDS法次之,高盐—低pH值法不适合。Comparison test was carried out on 3 separation methods of DNA from Parakmeria yunnanensis seed resulted that CTAB method was the best one, then SDS, and high salinity-low pH was not recommended. The methods could be applied in identification for seed purity of Parakmeria seed.

关 键 词：云南拟单性木兰 DNA 提取方法 种子 CTAB SDS RAPD 

分 类 号：Q946.2[生物学—植物学] Q949.747.1