维生素D致肥胖大鼠模型的实验研究 (英文)  

Experimental study on the obesity rat model induced by vitamin D

作　　者：李小林[1] 巫国辉[1] 谢琳[2] 文辉才[2] 刘诚[1] 刘善[1] 

机构地区：[1]江西省人民医院整形外科,江西省南昌市330006 [2]江西医学院研究生部,江西省南昌市330006

出　　处：《中国临床康复》2004年第9期1786-1787,共2页Chinese Journal of Clinical Rehabilitation

基　　金：江西省卫生厅中医药科研基金(2002A05)~~

摘　　要：目的:提供一种简易、经济、快速、便捷地诱发肥胖模型的方法。 方法:将雄性SD大鼠30只随机分为实验组和对照组,每组15只。实验组一次性腹腔注射维生素D300 U/g,对照组同时注射等量生理盐水。存活30 d后测Lee’s指数、脂肪系数、600倍光镜每视野脂肪细胞个数及血清瘦素水平。 结果:实验组Lee’s指数及脂肪系数(分别为313.7±6.9,0.26±0.05)明显高于对照组(分别为302.6±4.2,0.13±0.03)(t=5.352,9.455,P<0.05);实验组每视野脂肪细胞个数、血清瘦素水平[实验组:(31.2±17.6)μg/L,对照组:(7.5±6.3)μg/L]明显低于对照组(t=5.481,4.912,P<0.05)。 结论:一次性腹腔大剂量注射维生素D能方便快捷地诱发肥胖。AIM:To study a convenient and economical method of evoking obesity models. METHODS:Thirty male SD rats were randomly divided into experimental group and control group of 15 rats each.The rats in the experimental group were injected with vitamin D 300 U/g through abdominal cavity,while those in the control group with equivalent saline.Thirty days later all rats were killed,Lee' s index,fat coefficient and the number of adipocytes per sight under light microscope(600×),the level of leptin in serum were detected.RESULTS:It was showed the levels of Lee's index and fat coefficient were higher in the experimental group(313.7±6.9 and 0.26±0.05) than in the control group (302.6±4.2 and 0.13±0.03),(t = 5.352,9.455,P < 0.005);the number of adipocytes per sight under light microscope (600×) was reduced,while the leptin in serum increased [experimental group (31.2±17.6)(Ag/L,control group(7.5±6.3)u.g/L]. CONCLUSION:It is easy and convenient to evoke obesity by injecting large dosage vitamin D once through abdominal cavity.

关 键 词：维生素D 肥胖 大鼠 动物模型 实验 大剂量 

分 类 号：R977.24[医药卫生—药品] R589.2[医药卫生—药学]