细胞分离戊型肝炎病毒的初步研究  

Preliminary Report of Cell Culture of Hepatitis E Virus

作　　者：殷书荣[1] 刘玉堂[1] 田辛[1] 鲍作义[1] 蒋豫图[1] 张习坦[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国病毒学》1992年第2期134-137,共4页Virologica Sinica

摘　　要：本文报告用2BS细胞分离戊型肝炎病毒(HEV)感染成功的猴肝脏标本中的HEV的初步结果,将2BS细胞长成致密单层,接种已知含有HEV的肝悬液,盲传至第4代,发现在第27天细胞出现了轻微的细胞病变(CPE),随着进一步的传代,细胞病变时间逐渐提前。至第7代时,在第17天就可见到CPE,CPE的主要特征是细胞间隙变宽,透明度降低和轻度细胞破坏,无大片细胞溶解和脱落现象。免疫电镜检查在第5代和第6代细胞培养液中见到很少的27—34nm的病毒颗粒。将第4代至第7代的细胞悬液20ml接种了3只恒河猴,有2只猴在24天和26天出现了典型的ALT升高,待这3只猴恢复正常后一年,再次接种以上细胞悬液20ml,3只猴全部发生了ALT升高,并在胆汁中检查到27—34nm的病毒颗粒,我们初步推测2BS适应的这株病毒很可能是戊型肝炎病毒。Hepatitis E virus(HEV) was isolated in human embryomic lung cell inoculated with liver tissue suspension from an HEV-infected rhesus monkey, Slight cytopathic effect(CPE) appeared at the 27th day after 4 blind passages of viruses in cell culturs to at 17th day after 7 passages. Low amounts of 27—34nm virus-like particles(VLPs) in cell cultures were visualized by immunoelectronmicroseopy(IEM). 2 of 3 rhesus monkeys inoculated with 20ml of pool of cell cultures from 4 to 7 passages developed significant elevation of ALT levels and all of those monkeys inoculated with the same cell cultures one year after their recovery developed typical hepatitis. 27—34nm VLPs in their bile juice were found by IEM. It is concluded that those cell culture-adapted viruses may be hepatitis E virus.

关 键 词：戊肝病毒 细胞培养 

分 类 号：R373.21[医药卫生—病原生物学]