不同Ca^(2+)浓度对斑节对虾光感受器细胞内储存的Ca^(2+)的影响  

Effects of Different Calcium Concentration on Ca^(2+)in Ca^(2+)-Sequestering Organelles of Photoreceptor Cell in Penaeus Monodon Fabricius

作　　者：杜秀锦[1] 袁维佳[1] 张慧绮[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《上海师范大学学报（自然科学版）》2004年第1期86-91,共6页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市高等学校科学技术发展基金项目资助.

摘　　要：应用草酸 焦锑酸盐结合的沉淀技术研究了在不同Ca+2浓度条件下斑节对虾光感受器细胞胞内储存的Ca2+的变化,电镜观察表明:在高钙溶液培育后,细胞内的多囊体、色素颗粒、板膜体中都存在大量的焦锑酸钙沉淀的黑色颗粒,线粒体中则无;在生理溶液培育后,线粒体中出现沉淀,而其他Ca2+储存器中焦锑酸钙沉淀的黑色颗粒则大量减少.低钙溶液培育后焦锑酸钙沉淀的黑色颗粒情况与生理溶液条件下的相似.研究证实光感受器细胞内储存的Ca2+受到胞外Ca2+浓度的影响.By the combined oxalate-pyroantimonate technique, We observed the subcellular distribution of calcium in the photoreceptor cells of Penaeus monodon Fabricius with different the extracellular calcium concentration. The result showed that, in vitro, in presence of 50mmol/L, the quantity of calcium antimonate deposited in the multivesicular bodies, pigment and lamellar body were more than that of photoreceptor incubated in lower calcium solution, which contained 50mmol/L EGTA. In higher calcium solution, we could not find the deposit in the mitochondria, but in lower calcium solution, there was a little deposit in the mitochondria. The result of deposit distribution in the physiological saline was the same as lower calcium solution.

关 键 词：斑节对虾 光感受器 CA^2+ Ca^+储存器 

分 类 号：Q436[生物学—生理学]