烟草花叶病毒装配起始位点反义RNA表达型中间载体的构建  

Construction of Intermediate Vector Expressing Antisense RNA Gene for the Assembly Origin of TMV-RNA

作　　者：彭海[1] 沈学仁[1] 吴建华[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海20003

出　　处：《中国病毒学》1992年第4期426-431,共6页Virologica Sinica

摘　　要：利用基因工程方法培育抗病毒植物新品种的途径之一,是在植株中建立一个产生病毒基因组功能片段的反义RNA的系统。本工作设计并合成了一段烟草花叶病毒(TMV)装配起始位点反义RNA的基因,再以pBR 325为基本质粒,构建了包含带有花椰菜花叶病毒(CaMV)35S启动子和PolyA信号的反义RNA表达单元,以及为筛选转基因植株所必须的NPT-Ⅱ表达单元的中间载体,为以后经土壤农杆菌而获得转基因烟草植株打下了基础。In this paper we have designed and constructed an antisense gene which is complementary with the assembly origin of TMV-RNA, then a intermediate vector which contains the expressing unitgconsisting of CaMV 35S pro. moter, antisense RNA gene and poly A signal, as well as the NPT-Ⅱ expressing unit was constructed, The NPT-Ⅱ is essential for the selection and transformation of plants, Thus, the successful construction of this interme diate vector will faciliate obtaining the transgenic plants.

关 键 词：烟草花叶病毒 反义RNA 中间载体 

分 类 号：S432.41[农业科学—植物病理学]