抗人载脂蛋白E单克隆抗体制备及免疫学特性的研究  

Preparation and analysis of immunological property of monoclonal antibodies against human apolipoprotein E

作　　者：张东升[1] 夏辉明[1] 赵明跃 吕新跃[1] 夏福臻[1] 刘守彦[1] 

机构地区：[1]河南医科大学病理生理教研室

出　　处：《中国病理生理杂志》1992年第3期229-233,共5页Chinese Journal of Pathophysiology

摘　　要：应用人血清纯化的ApoE免疫BALB/C小鼠,其脾细胞与NS-1骨髓瘤细胞融合,获得3株分泌抗人ApoE单克隆抗体的杂交瘤细胞株(E6C3,E4C2和E3C3),对其免疫学特性进行了研究。腹水效价为8×10^(-5)～2×10^(-6),与其它载脂蛋白没有交叉反应,制备免疫亲和层析柱纯化了ApoE,单抗相加试验和双抗体夹心试验证明,两株MeAb识别ApoE的两个不同抗原决定簇,抗体亚型均为IgG_1型。应用ELISA双抗体夹心法测定了人血清ApoE含量。Apolipoprotein E (ApoE) was purified from human serum and used as the antigen to immunize BALB/C mice. The splenic cells of the mice fused with NS-1 mouse myeloma cells. Three hybridoma cell lines(E3C3, E6C3 and E4C2)secreting anti-human ApoE McAb were established. The immunological property of the McAb was studied. The ascites fluids titer were 8×10^(-5)2×10^(-6). The McAbs did not cross react with the other apolipoprotins. ApoE was purified by immunoaffinity chromatography prepared by using the ApoE McAb. McAbs recognized two distinct respective epitopcs on ApoE. The subclass of immunoglabulin of three McAbs were IgG_1. A double McAb sandwich EL1SA was developed to evaluate ApoE levels in normal and hyperlipidemia human serum.

关 键 词：单克隆抗体 杂交瘤 载脂蛋白E 

分 类 号：R392.11[医药卫生—免疫学]