大口径毛细管柱气相色谱法测定鱼油软胶囊中EPA-E和DHA-E的含量  

Determination of eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester in the fish oil capsule by 530 fun capillary GC

作　　者：邓亮[1] 叶利民[1] 陈聪[1] 李章万[1] 张学容[1] 姚倩 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]深圳海王药业有限公司,广东深圳518054

出　　处：《华西药学杂志》2004年第2期117-119,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的 测定鱼油软胶囊中二十碳五烯酸乙酯(EPA-E)和二十二碳六烯酸乙酯(DHA-E)的含量。方法 采用530 μm大口径毛细管柱气相色谱法,外标法测定EPA-E和DHA-E的含量。结果 EPA-E和DHA-E的保留时间分别为8.0 min和12.0 min,其标准曲线分别在0.52～1.22μg和0.58-1.36μg之间有良好的线性关系,回收率分别为97.1％和98.2％。结论 该方法专属性强,适用于鱼油软胶囊中EPA-E和DHA-E的定量分析。OBJECTIVE To establish a 530 fan capillary gas chromatography method for the determination of eicosapentaenoic acid ethyl ester (EPA - E)and docosahexaenoic acid ethyl ester(DHA - E) in fish oil capsules.METHODS Samples were determined by 530 μm capillary gas chromatography with external standardization.RESULTS The retention time of EPA - E and DHA - E were 8.0 min and 12.0 min, respectively. The standard curves of EPA - E and DHA - E were linear in the range 0.52 to 1.22 μg and 0.58 to 1.36 μg, respectively. The average recoveries were 97.1 % for EPA - E and 98.2 % for DHA - E. CONCLUSION The method was sensitive and specific. It was suitable for analysis of EPA - E and DHA - E in fish oil capsules.

关 键 词：大口径毛细管柱气相色谱法 鱼油软胶囊 EPA-E DHA-E 含量测定 质量控制 

分 类 号：R927.2[医药卫生—药学]