RP-HPLC测定血浆中乌拉地尔的浓度  

Determination of Urapidil in plasma by RP - HPLC

作　　者：周静[1] 蒋学华[1] 程强[2] 林舒[1] 杨俊毅[1] 金朝辉[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川抗菌素工业研究所,四川成都610051

出　　处：《华西药学杂志》2004年第2期119-120,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的 建立测定受试者口服乌拉地尔缓释胶囊后乌拉地尔血药浓度的方法。方法 采用RP-HPLC法,以1,3-二甲基-4-(γ-氯丙基氨基)-尿嘧啶为内标,乙腈-0.05 mol·1-1KH2PO4溶液-三乙胺-冰乙酸(20∶80∶0.1∶0.1)为流动相,检测波长270 nm。结果 血浆中乌拉地尔的平均回收率为98.9％±5.15％,日内RSD<7.37％,日间RSD<12.5％;在室温条件下乌拉地尔血浆样品至少能稳定8 h,在冰冻条件下至少能稳定30 d。结论 方法准确、简便、重复性好。OBJECTIVE To establish an RP - HPLC method for the determination of Urapidil in plasma from 24 healthy male volunteers who have been treated with Oral Urapidil sustained - release capsules. METHODS Samples were determined by RP - HPLC. The condition was acetonitril - 0.05 mol·l-1KH2PO4- triethylamine- glacial acetic acid(20:80:0.1:0.1) as the mobile phase and 270 nm as the detective wavelength. RESULTS The average recovery for Urapidil in plasma was 98.9%±5.15%. Within - day and between - day precision( RSD) were less than 13% .The plasma samples of Urapidil was stable within 8 hours at room temperature and within 30 days by freezing. CONCLUSION The method is accurate,simple and well reproducible.

关 键 词：RP-HPLC 乌拉地尔 血药浓度 样品处理 色谱条件 标准曲线 

分 类 号：R969[医药卫生—药理学] R972.4[医药卫生—药学]