RP-HPLC测定犬血浆中3种丹参酮的浓度  

Determination of three kinds of Tanshinone in dog plasma by RP - HPLC

作　　者：李莉[1] 蒋学华[1] 任静[1] 陈卓[1] 余海云[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2004年第2期121-123,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然基金资助项目(批准号:30070935和30271614)

摘　　要：目的 建立RP-HPLC法同时测定家犬血浆中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ的含量。方法 采用DiamonsilTM C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(89∶11),流速0.7 ml·min-1,检测波长为254 nm,温度为室温,血浆样品加内标吉非罗齐,甲醇沉淀蛋白,上清液进样。结果 家犬血浆中内源性成分对3种丹参酮的测定无干扰;丹参酮ⅡA在0.08-6.3μg·ml-1浓度范围内呈良好的线性关系(r=0.9994),平均方法回收率为99.5％;隐丹参酮在0.08-8.0μg·ml-1浓度范围内呈良好的线性关系(r=0.9996),平均方法回收率为100.6％;丹参酮Ⅰ在0.10-6.0μg·ml-1浓度范围内呈良好的线性关系(r=0.9996),平均方法回收率为102.5％。3种丹参酮测定的日内RSD在0.75％～4.43％之间,日间RSD在2.8％2～7.65％之间。结论 该法样品处理简便,灵敏度较高,结果准确。OBJECTIVE To establish a sensitive and reliable RP - HPLC method for the determination of Tanshinone ⅡA(TSⅡA),Cryp-totanshinone(CT) and Tanshinone Ⅰ (TSⅠ )in dog plasma. METHODS Concentrations of Tanshinones in plasma were measured by RP-HPLC after pretreatment with Gemifibrozil methanol solution to precipitate the protein. Separation and determination were achieved on a Dia-monsil?ODS C18 column (4.6 mm×250 mm, 5 μm) . The mobile phase consisted of methanol - water ( 89:11) at a flow rate of 0.7 ml· min-1. Tanshinones were monitored at a wavelength of 254 run at room temperature. RESULTS Three Tanshinones were separated and no interference was found. Good liner relationships of TSⅡA,CT and TSⅠ were separately obtained between 0.08 to 6.3 μg· ml-1( r = 0.9994) , between 0.08 to 8.00 μg·ml-1( r = 0.99%) .between 0.10 to 6.00 μg·ml-1( r = 0.99%) .The mean recoveries were 99.5% , 100.6% and 102.5% in sequence.The within a day RSD were between 0.75% to 4.43% and the between days RSD were between 2.82% to 7.65% . CONCLUSION This method is simple, rapid, sensitive, accurate with a wide linear range and suitable for the analysis of Tanshinones - containing plasma samples.

关 键 词：RP-HPLC 血浆 丹参酮 药物浓度 动物实验 样品处理 色谱条件 

分 类 号：R965[医药卫生—药理学]