次声对视网膜微血管内皮细胞胞浆[Ca^(2+)]i水平的影响  

The Study of Cytoplasmic Free Calcium Concentration of the Bovine Retinal Microvessel Endothelium Cells Under Infrasound Exposure

作　　者：邱萍[1] 李泱[1] 温宁[2] 姜勇[3] 张作明[4] 王士雯[1] 

机构地区：[1]解放军总医院老年心血管病研究所,北京100853 [2]解放军总医院口腔科,北京100853 [3]第一军医大学病理生理教研室 [4]第四军医大学空军医学系航空临床教研室

出　　处：《中国微循环》2004年第2期79-82,共4页Journal of Chinese Microcirculation

基　　金：国家自然科学基金(No.39900162);军队九五重点课题资助(No.96Z042);国家博士后科学基金资助(No.2003033189)

摘　　要：目的通过检测次声暴露前后视网膜微血管内皮细胞(BREC)胞浆[Ca2 +]i水平的改变 ,对次声致大鼠血 -视网膜屏障通透性改变的细胞内信号转导途径进行初步探讨。方法采用荧光探针Flu-3研究次声及BKCa 开放剂NS -1619干预下BREC胞浆[Ca2 +]i水平的改变。结果次声暴露导致细胞内钙库释放Ca2 +,同时观察到NS-1619外钙依赖性地增加[Ca2 +]i。结论次声引起[Ca2 +]i升高 ,通过细胞收缩成份引起内皮细胞主动收缩 ,从而达到对血 -视网膜屏障的调节效应。Objective To further understand the mechanism of the change that infrasound brings to BRB.Laser scanning confocal microscopy(LSCM)was used to detect the change of cytosolic free calcium[Ca 2+ ]i in the bovine retinal microvessel endothelium cells(BREC)under infrasound exposure.Methods The change of[Ca 2+ ]i under the infrasound exposure and NS-1619was studied by fluorescent indicator Flu-3.Results Pure exposure to infrasound can result in calcium release from the calcium store in the cell.Meanwhile,the increase of[Ca 2+ ]i under NS-1619is also observed.Conclusions The infrasound may result in the increase of[Ca 2+ ]i and through calcium-dependent interaction of endothelium,the adverse effect on BRB is achieved thereby.

关 键 词：视网膜 微血管内皮细胞 次声 激光共聚焦显微镜 胞浆内游离钙离子浓度 钙释放 细胞内信号转导 

分 类 号：R339.146[医药卫生—人体生理学] Q257[医药卫生—基础医学]