高糖对视网膜Müller细胞碱性成纤维细胞生长因子表达及钙离子通道的影响  

Effects of high concentration glucose on bFGF expressionand calcium channels of retinal Müller cells cultured in vitro

作　　者：冷瀛[1] 王溪原[2] 王越晖[3] 张文杰[4] 张晓光[1] 

机构地区：[1]吉林大学第二医院眼科,吉林长春130041 [2]沈阳铁路局中心医院,辽宁沈阳110023 [3]吉林大学病理生物学教育部重点实验室,吉林长春130021 [4]吉林大学基础医学院生理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2004年第2期195-197,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅基金资助项目 (990 5 75 - 5 )

摘　　要：目的 :观察在高浓度葡萄糖条件下体外培养兔视网膜 Müller细胞碱性成纤维细胞生长因子 (basicfibroblast growth factor,b FGF)的表达及其钙 (Ca2 + )通道的变化。方法 :采用免疫细胞化学及透射电镜方法鉴定体外培养的兔视网膜 Müller细胞 ;应用免疫细胞化学方法定性观察高糖条件下视网膜 Müller细胞 b FGF表达的变化 ;利用膜片钳技术观察高糖条件下 Müller细胞 Ca2 + 通道的变化。结果 :高糖可以刺激视网膜 Müller细胞表达b FGF,对 Ca2 + 通道无明显影响。结论 :高糖刺激 Müller细胞 b FGF的表达 ,而发挥促血管生成的作用 ,在此过程中 Ca2 +ObjectiveTo observe the effects of high concentration glucose on bFGF expression and calcium channels of rabbit retinal Müller cells cultured in vitro . MethodsTransmission electron microscopy and immunocytochemical staining method were used to identify Müller cells. The changes of bFGF expressed by Müller cells were studied with immunocytochemical method in a qualitative way. Patch clamp technique was used to observe the changes of calcium channels caused by high concentration glucose. ResultsbFGF expression was enhanced obviously by high concentration glucose. Obvious changes of calcium channels were not found. ConclusionbFGF expression secreted by cultured Müller cells in vitro can be enhanced at high concentration glucose. Müller cells play a potential role in neovascularization of diabetic retinopathy. Obvious changes of calcium channels are not found during the process.

关 键 词：葡萄糖溶液 高渗 成纤维细胞生长因子2 MUELLER细胞 视网膜疾病 糖尿病 钙通道 

分 类 号：R339.146[医药卫生—人体生理学] R774.1[医药卫生—基础医学]