放线共生放线杆菌的分离、鉴定及耐药谱的测定  

Identification of Actinobacillus actinomycetemcomitans and its spectrum of resistance to antibiotics

作　　者：李毅[1] 杨涛[2] 孙延波[3] 关显智[3] 黄洋[1] 孙宏晨[1] 

机构地区：[1]吉林大学口腔医院口腔内科,吉林长春130041 [2]长春市儿童医院口腔科,吉林长春130051 [3]吉林大学基础医学院病原微生物学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2004年第2期227-229,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科研发展计划项目 (2 0 0 1 0 5 1 9)

摘　　要：目的 :探讨放线共生放线杆菌 (A.a)分离、鉴定的方法 ,确定其耐药谱。方法 :采用选择性培养基、厌氧培养技术及生化鉴定的方法从临床青少年牙周炎、成人快速进展型牙周炎及青少年严重牙龈炎患者的牙周袋内分离出 A.a。在此基础上进行了 1 0种抗生素对所分离的全部 A.a菌株及 1株标准菌株最低抑菌浓度的检测。结果 :共分离出 2 4株 A.a,其对庆大霉素、四环素、氨苄青霉素、丁氨卡那霉素及青霉素 G呈现出不同程度的耐药性 ,但对罗红霉素、甲硝唑、链霉素、红霉素及头孢唑啉钠敏感。结论 :罗红霉素、甲硝唑、链霉素、红霉素及头孢唑啉钠在治疗与 A.a相关的牙周病能够发挥作用 ,本研究为 A.ObjectiveTo study the method to isolate and identify Actinobacillus actinomycetemcomitans (A a) and insure its resistance to antibiotics. Methods A a was isolated from specimens of human periodontal disease, and identification of A a was carried out by anaerobic cultivation, observation of growth properties on TSBV and systematically biochemical reaction; and the spectrum of A a ′s resistance to antibiotics was identified. ResultsTwenty four strains of A a were acquired and identified; They were sensitive to Re,MTZ,Sm,Em,and Cef. ConclusionRe,MTZ,Sm,Em, and Cef can play a important role in treatment of peridontitis.

关 键 词：放线共生放线杆菌 抑菌浓度 

分 类 号：R379.1[医药卫生—病原生物学]