蛋白激酶C同工酶β、δ与糖尿病视网膜病变关系的实验研究  

Experimental study on the relationship between protein kinase C isoforms β、δ and diabetic retinopathy

作　　者：周波[1] 汪恕萍[1] 曾昭淳[2] 

机构地区：[1]重庆医科大学临床学院内分泌科 [2]重庆医科大学基础医学院生化研究室,重庆400016

出　　处：《重庆医科大学学报》2004年第2期121-123,137,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生局科研基金资助 ( 0 0 -2 0 2 6)

摘　　要：目的 :探讨糖尿病视网膜病变 (DR)发病过程中视网膜血管蛋白激酶C(PKC)同工酶 β、δ表达变化及其与DR发生、发展的关系。方法 :将实验动物随机分为正常对照组及链脲菌素诱导的糖尿病组。采用渗透休克法获取大鼠视网膜血管。在成模后 2周、2月、4月、6月 ,采用Westernblot法检测视网膜血管PKC同工酶 β、δ表达情况。毛细血管床形态立体定量评估DR。结果 :PKC - β在糖尿病大鼠视网膜血管 2周、2月、4月、6月时呈胞膜至胞浆部移位激活 ,而PKC -δ在糖尿病大鼠视网膜血管 2周、2月、4月、6月时呈胞膜至胞浆部移位激活。成模 6月后 ,视网膜深层毛细血管呈现周细胞减少、基底膜增厚的特征性改变 ,而成模 4月时 ,电镜显示糖尿病大鼠深层毛细血管基底膜增厚。结论 :在DR发病过程中的不同阶段 ,PKC同工酶 β、δ在视网膜血管的变化不同 ,对DR的发生、发展有着不同的调控效应。Objective:To investigate the expression of protein kinase C (PKC) isoforms β,δ in diabetic rats and evaluate the relation between altered PKC β,δ expression and the pathogenesis of diabetic retinopathy (DR).Methods:Wistar rats were randomly divided into control group and diabetic group induced by streptozotocin;osmotic shock method was used to isolate the retinal vessels of rats;and PKC isoforms β,δ espression were determined after 2 weeks,2 months,4 months and 6 months follw-up.DR was assessed by quantitative morphometry of capillary bed.Results:Diabetes could induce the translocation of PKC isoform β from cytosol to membrane pool after 2 weeks,2 months,4 months and 6 months,but δ isoform translocated from membrane to cytosol pool in retinal vessels of diabetic rats after 2 weeks,2 months,4 months and 6 months.Diabetic rats showed pericyte decrease and capillary basement thickening in deep capillary bed of retina after 6 months follow-up.Conclusion:The expression of PKC isoforms β,δ varies in different stages of DR,and the effect of PKC isoforms on the pathogenesis of DR is different.

关 键 词：蛋白激酶C 同工酶 视网膜 糖尿病 

分 类 号：R797.9[医药卫生—临床医学]