吸烟与非吸烟者气道纤毛轴动力蛋白基因DNAI1的表达差异  

Expression differences of axonemal dynein gene DNAI1 of airway between smokers and non-smokers

作　　者：张郁[1] 陈良安[1] 田庆[1] 范保星[1] 刘又宁[1] 

机构地区：[1]解放军总医院呼吸科,北京市100853

出　　处：《中国临床康复》2004年第12期2284-2285,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:检测纤毛轴动力蛋白基因DNAI1在吸烟者和非吸烟者支气管组织的表达差异,了解吸烟对纤毛轴动力蛋白基因的影响。方法:分别提取8例吸烟者和8例非吸烟者的支气管组织总RNA,用反转录聚合酶链反应方法分析纤毛轴动力蛋白基因DNAI1在上述组织的表达水平。结果:吸烟者和非吸烟者DNAI1的表达水平分别为0.35±0.02和0.60±0.07,两者相比有显著差异(t=9.96,P<0.01)。结论:纤毛轴动力蛋白基因DNAI1在吸烟者支气管组织中的表达明显降低,可能是吸烟造成纤毛运动功能下降的原因之一。AIM:To detect the expression difference of axonemal dynein gene DNAI1 in bronchial tissues between the smoker and the non smoker,and to explore the effect of smoking on axonemal dynein gene. METHODS: Total RNAs in bronchial tissues were extracted from 8 smokers and 8 non smokers respectively, the expression level of DNAI1 was analyzed by reverse transcription polymerase chain reaction(RT PCR). RESULTS:The expression level of DNAI1 was 0.34±0.02 for smokers, significantly different from that of non smokers(0.60±0.07,t=9.96,P< 0.01).The expression of axonemal dynein gene DNAI1 in bronchial tissues of smoker significantly decreased,which may be one of the reasons for the decrease of cilium movement caused by smoking.

关 键 词：吸烟 气道 纤毛轴动力蛋白基因 DNAI1 基因表达 检测 支气管组织 

分 类 号：R163[医药卫生—公共卫生与预防医学] R394