血小板第四因子对脐血CD34^+细胞趋化作用的研究  

THE EFFECT OF PLATELET FACTOR 4 ON THE CHEMOATTRACT FUNCTION OF THE CORD BLOOD CD34+ CELLS

作　　者：冯义[1] 卢士红[1] 宋国丽[1] 张志华[1] 韩忠朝[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所国家重点实验室

出　　处：《中国应用生理学杂志》2003年第2期141-144,共4页Chinese Journal of Applied Physiology

基　　金：攀登计划项目 ( 95 - 专- 10 ) ;天津市科技重大公关资助项目 ( 0 0 3114 311)

摘　　要：目的 :研究PF4及其小肽PF417 70对新鲜脐血CD34+细胞的趋化作用及对粘附分子表达的影响。方法 :采用免疫磁珠法 (MACS)分选CD34+细胞 ,利用Transwell穿孔板测定PF4对CD34+细胞的趋化作用 ;流式细胞仪检测免疫荧光标记的粘附分子及CXCR4的表达。结果 :①PF4对脐血CD34+细胞有趋化作用 ,PF4组的趋化百分比为 15 7.43%± 5 0 .0 6 %(P <0 .0 5 ) ,PF417 70组为 187.0 2 %± 10 .6 9%(P <0 .0 5 )。②PF4作用于CD34+细胞时 ,CD49d和CXCR 4表达增加 ,对其它粘附分子CD31,CD44 ,CD11a ,CD6 2 p ,CD6 2E的表达没有影响。 结论 :PF4对脐血CD34+细胞有趋化作用 ,促进整合素CD49d及CXCR4的表达 ,PF4有助于脐血干细胞的归巢。Aim: To study the effect of PF4 and relative peptide PF4 17-70 on the chemoattract ability, the expression of adhesion molecules and CXCR4 on the flesh cord blood CD34+ cells. Methods: CD34+ cells were separated from the cord blood using MACS immune magnetic beads, the chemoattract ability was assayed using the Transwell board, the expression of adhesion molecules and CXCR4 was measured by FACS. Results: ① PF4 and PF4 17-70 increased the migration of the CD34+ cells, the chemoattract percentage of PF4 was 157.43%±50.06%(P<0.05) and PF4 17-70 was 187.02%±10.69%(P<0.05). ② The expression of CD49d and CXCR4 on the CD34+ cells increased after PF4 incubated, but the expressions of other adherent molecules including CD31, CD44, CD11a, CD62p, CD62E did not change. Conclusion: PF4 has the chemoattract ability on the umbilical blood CD34+ cells by promoting the expression of integrin CD49d and CXCR4, PF4 may help the cord stem cells homing.

关 键 词：血小板第四因子 脐血 CD34^+细胞 趋化作用 粘附分子 CB 造血干细胞 

分 类 号：Q254[生物学—细胞生物学] R331[医药卫生—人体生理学]