氟对成骨细胞样细胞胞内钙和钙通道电流的影响  

EFFECTS OF FLUORIDE ON IONIZED CALCIUM AND CALCIUM CHANNEL IN OSTEOBLAST-LIKE CELL IN VITRO

作　　者：华坤[1] 赵红[2] 黄民[3] 李广生[1] 

机构地区：[1]吉林大学再生医学研究所病理研究室,吉林长春130021 [2]吉林大学中日联谊医院中心研究室,吉林长春130031 [3]吉林大学白求恩医学部生理教研室,吉林长春130021

出　　处：《中国应用生理学杂志》2003年第2期179-181,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目 ( 39730 390 )

摘　　要：目的 :观察氟 (Fluoride,F-)对培养的成骨细胞样细胞胞内钙和钙通道电流的影响。方法 :应用Fura 2负载的成骨细胞样细胞 ,通过紫外荧光分光光度仪测定细胞内游离钙的浓度 ,同时应用全细胞膜片钳技术记录成骨细胞样细胞钙通道的变化。结果 :氟可引起成骨细胞样细胞胞内钙增高 ,尤以 10 0ng/mlF-最为明显 ,与对照组比较差异显著 (P <0 .0 5 )。应用全细胞膜片钳技术发现 2 5ng/mlF-即可引起成骨细胞样细胞钙通道开放 ,随着染F-剂量的增加 ,Ca2 +通道电流的幅值增大 (P <0 .0 1) ,且F-对Ca2 +通道电流的兴奋作用呈剂量依赖性 (r =0 .9914)。结论 :F-可引起成骨细胞样细胞钙通道开放 ,从而导致细胞胞内钙浓度的增加。Aim: To study the effects of fluoride (F-) on ionized calcium ( i) and calcium channel in osteoblast-like cell(OBLs). Methods: Invistigating i was by fluorospectrophotometry and recording the calcium currents of OBLs was using whole patch-clamp technique. Results: The addition of fluoride to the medium made a rapid and significant increase in free i , especially in 100 ng F-/ml group. There were significances between control group and 50,100 ng F-/ml groups. And 25 ng/ml F- could stimulate OBLs calcium channel open. Compared with control group, fluoride could significantly increase the amplitudes of calcium currents (P<0.01), furthermore the activation was in dose-dependent manner. Conclusion: Fluoride can make calcium channel open and then increase the concentrations of i.

关 键 词：氟化物 成骨细胞 钙 膜片钳 通道 电流 氟骨症 

分 类 号：R336[医药卫生—人体生理学]