辣椒素敏感传入神经和NO中介大鼠胃扩张引起的胃酸分泌和胃粘膜血流量变化  

CAPSAICIN-SENSITIVE AFFERENT FIBERS AND ENDOGENOUS NO MEIDATE THE GASTRIC ACID SECRETION AND GASTRIC MUCOSAL BLOOD FLOW IN INTRAGASTRIC DISTENTION IN RATS

作　　者：顾洛[1] 闫长栋[2] 杜军[1] 田苏平[1] 李栋生[1] 

机构地区：[1]南京医科大学生理学系,江苏南京210029 [2]徐州医学院生理学教研室,江苏徐州221002

出　　处：《中国应用生理学杂志》2003年第2期193-196,共4页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :本文采用氢气清除法测定胃粘膜血流量以及大剂量辣椒素使传入神经失去功能的技术 ,观察大鼠胃扩张过程中引起胃酸分泌和胃粘膜血流量 ( gastricmucosalbloodflow ,GMBF)的变化以及传入神经和内源性NO在这一效应中的作用。结果 :①大鼠胃扩张引起胃酸分泌时GMBF增加。②预先用大剂量辣椒素消除传入神经作用可阻断胃扩张引起的GMBF增加效应 ,并部分阻断胃酸分泌。③预先静脉注射一氧化氮 (nitricoxide ,NO)生物合成阻断剂L nitro L argininemethylester (L NAME)胃扩张引起的GMBF增多效应消失 ,同时胃酸分泌减弱。结论 :辣椒素敏感传入神经和内源性NO参与胃扩张引起的胃酸分泌及胃粘膜血流增多效应。Aim and methods: By hydrogen gas clearance technique to measure gastric mucosal blood flow (GMBF) and a high dose of capsaicin to ablate the capsaicin-sensitive afferent fibers, the roles of capsaicin-sensitive afferent fibers and endogenous NO in the gastric acid secretion and hyperemic response to intragastric distention were studied in rats. Results: ①There was an increase in acid secretion associated with the increase in GMBF to intragastric distention. ②Pretreatment with a high dose of capsaicin to ablate afferent fibers completely abolished the GMBF and partially inhibited the acid secretion during the intragastric distention. ③The increase in GMBF to intragastric distention was completely blocked by pretreatment with L-NAME, whereas the acid secretion was significantly attenuated. Conclusion: Capsaicin-sensitive afferent fibers and endogenous NO are involved in the increases of gastric acid secretion and GMBF.

关 键 词：一氧化氮 NO 辣椒素敏感传入神经 胃酸 L-NAME 大鼠 胃扩张 胃粘膜血流量 

分 类 号：Q425[生物学—神经生物学] Q482[生物学—生理学]