胶质芽胞杆菌(Bacillus mucilaginosus)D_4B_1菌株生理特性研究  

Studies on physiology of Bacillus mucilaginosus D_4B_1 strain

作　　者：吴作为[1] 吴颖运[2] 李欣[3] 晏晶[3] 张克勤[1] 

机构地区：[1]云南大学省工业微生物发酵工程重点实验室,昆明650091 [2]南开大学生命科学学院,天津3000713 [3]南开大学分子生物学研究所,天津300071

出　　处：《土壤肥料》2004年第2期40-43,共4页Soils and Fertilizers

基　　金：云南省省校合作项目:"新型植酸酶工程菌肥研制与开发"(2001GBBFAO2H019);国家863项目"多功能(解磷;解钾)基因工程的构建及应用"(2002AA213061)。

摘　　要：对胶质芽孢杆菌D4B1菌株生理特性研究表明,D4B1菌株在供试培养基A中生长良好,12h进入对数生长期,48h达到生长高峰,菌体浓度为6 0×108个/mL;对氨苄青霉素、卡那霉素、氯霉素、硫酸链霉素没有抗性;能分泌胞外活性物质抑制植物病原真菌的生长;具有较强的解磷解钾能力,但无植酸酶活性。其结果可以作为进一步分子改造胶质芽胞杆菌的参考。Bacillus mucilaginosus D_4B_1 strain growN exponentially after it was inoculated into Medium A for about 12hs,and the concentration of culture amounted to 6.0×10~8 /mL at 48h. It showed no antibiotic resistance to Ampicillin,Kanamycin,Chloramphenicol and Streptomycin Sulfate. Fungi which caused disease to plants could be inhibited by its excreting bioactive substances exocellularly. Bacillus mucilaginosus D_4B_1 was also capable of releasing potassium and phosphorus but had no phytase activity. The results in this paper would be references to engineering Bacillus mucilaginosus by molecular methods.

关 键 词：胶质芽胞杆菌 D4B1菌株 培养基 生理特性 菌体浓度 微生物肥料 

分 类 号：S144[农业科学—肥料学]