抗马拉硫磷褐飞虱品系的交互抗性及其生化分析  

Cross resistance of malathion resistant strain of Nilaparvata lugens and its biochemical aspects

作　　者：刘泽文[1] 韩召军[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2004年第1期55-58,共4页Journal of Nanjing Agricultural University

基　　金：江苏省重点攻关项目 (BE2 0 0 13 45 ) ;江苏省自然科学基金项目 (BK19990 5 9)

摘　　要：用马拉硫磷筛选的褐飞虱抗性品系 (R)对马拉硫磷的抗性达到 1 84 4倍 ,对作用于乙酰胆碱酯酶的二嗪磷、异丙威和仲丁威有一定的交互抗性 ,而对氰戊菊酯不表现显著的交互抗性。进一步研究显示 :在抗性品系中 ,磷酸三苯酯对马拉硫磷和仲丁威的增效作用分别达到 9 1 5和 2 38倍 ,顺丁烯二酸二乙酯对这两个药剂也有一定的增效作用 (2 32和 1 4 2倍 )。抗性品系的酯酶、谷胱甘肽 S 转移酶 (以 2 ,4二硝基氯苯为底物 )和多功能氧化酶活力分别为敏感品系(S)的 1 4 6 7、 1 5 2和 1 1 1倍。在抗性品系中 ,磷酸三苯酯对酯酶活力抑制作用很强 ,顺丁烯二酸二乙酯对谷胱甘肽 S 转移酶活力也存在一定的抑制作用。实验结果显示 ,酯酶和谷胱甘肽 SThe resistant strain(R)of brown planthopper selected by malathion held 184 4 fold resistance to malathion and showed obvious cross resistance to all the acetylcholine receptor targeting insecticides tested,but not to fenvalerate.Further study demonstrated that TPP had significant synergism on malathion(SR:9 15)and some on fenobucarb(SR:2 38),and DEM had some synergism to malathion(SR:2 32)and to fenobucarb(SR:1 42)in R strain.Biochemical assay showed that R strain had esterase activity 14 67 times that in susceptible strain(S),GST with CDNB 1 52 and MFO only 1 11.And TPP could significantly inhibit the esterase activity in R strain,and DEM also could inhibit the GST activity to some extent in R strain.It is concluded that the esterase and GST played an important role in the malathion resistance and the cross resistance to fenobucarb to different extents.

关 键 词：马拉硫磷 褐飞虱 抗性品系 交互抗性 增效作用 酯酶 谷胱甘肽-S-转移酶 多功能氧化酶 杀虫剂 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] S482.3[农业科学—植物保护]