猪繁殖与呼吸综合征病毒疫苗不同接种途径对母猪生殖道黏膜淋巴细胞分布的影响  

Effect of immune ways of PRRSV attenuated vaccine on the distribution of lymphocyte in sow genital tract

作　　者：金光明[1] 刘胜兵[1] 杨倩[1] 李玉磊[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《南京农业大学学报》2004年第1期124-126,共3页Journal of Nanjing Agricultural University

基　　金：江苏省自然科学基金资助项目 (BK2 0 0 2 111)

摘　　要：16头 5月龄长白×大约克夏二元杂交母猪 ,通过不同途径接种猪繁殖与呼吸综合征病毒 (PRRSV)弱毒疫苗 ,采用组织学方法研究其对母猪生殖道黏膜淋巴细胞分布的影响。结果表明 ,与对照组相比 ,鼻腔和肌注接种可使猪子宫角、子宫体和子宫颈黏膜上皮内淋巴细胞的数量极显著增加 (P <0 0 1 ) ,生殖道接种则使淋巴细胞的数量极显著减少(P <0 0 1 )。鼻腔与肌注接种间无明显差异。经生殖道接种疫苗时 ,子宫黏膜上皮变得不规则 ,有些部位上皮变薄。而经鼻腔和肌注接种时 ,黏膜固有层中多见淋巴细胞聚集 ,形成淋巴细胞群 ,并向黏膜腔表面排放。Sixteen 5 month old sows of Landrace×Large Yorkshire crossbred were immunized by porcine reproductive and respiratory syndrome virus(PRRSV)attenuated vaccine on the ways of nasal,genital and intramuscular injection.The effect of immune ways on the intraepithelial lymphocyte(iIEL)distribution of genital tract was investigated by histological method.The results showed that the number of iIEL in the uterus of sows inoculated through nasal and intramuscular injection sighificantly increased than that of control group( P< 0 01).The number of iIEL in the uterus of sows inoculated through genital mucosa sighificantly decreased than that of control group( P <0 01).The number of iIEL in the uterus had no significant difference among the nasal and intramuscular routs.After PRRSV attenuated vaccine Resp was inoculated by genital mucosa,the epithelium presented shorter.The lymphocytes in propria lamina of uterus were gathered in colony,which passed through the epithelium and arrived the epithelial surface.The results showed that the different immunized ways of PRRSV attenuated vaccine had significant effect on the distribution and number of iIEL in uterus and histological structure.

关 键 词：猪 繁殖呼吸综合征病毒 疫苗接种途径 生殖道黏膜 淋巴细胞 分布 

分 类 号：S858.28[农业科学—临床兽医学] S852.52[农业科学—兽医学]