应用原生质体融合技术获得茄子种间体细胞杂种  

Using Protoplast Electrofusion Technology to Obtain Interspecific Somatic Fusion Hybrid of Eggplant

作　　者：连勇[1] 刘富中[1] 冯东昕[1] 宋燕[1] 陈钰辉[1] 张松林[1] Darasinh Sihachakr 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]法国巴黎11大学植物形态与发育实验室

出　　处：《园艺学报》2004年第1期39-42,共4页Acta Horticulturae Sinica

基　　金：国家‘8 63’资助项目 ( 2 0 0 2AA2 44 0 11-1) ;欧盟资助项目 (ICA4 CT 2 0 0 1 10 0 64 )

摘　　要：应用原生质体电融合技术 ,获得了茄子近缘野生种Solanumtorvum、S aethiopicum与栽培种S melongena (六叶茄 ,Dourga)的种间体细胞融合四倍体再生植株。染色体检测表明该植株为四倍体 (2n =4x =4 8) ,荧光免疫检测证明为种间体细胞杂种 ,青枯病抗性鉴定表明带有抗病基因 。We obtained regenerated tetroaploid plants from the protoplast electrofusion between Solanum melongena(Dourga)and S.torvum or S.aethiopicum.The ploid level was confirmed by chromosome detection.Fluorescent immunoassay proved that the plants were regenerated from interspecific somatic fusion.Inoculation with Pseudomonas solanacearum indicated that the somatic hybrids carried the resistance gene from the wild relatives.The somatic hybrids were more like the wild relatives in the open field.

关 键 词：原生质体融合技术 茄子 种间杂交 体细胞杂种 青桔病 抗病基因 

分 类 号：S641.1[农业科学—蔬菜学]