Polymerization of o-Phenylenediamine Catalyzed byHemeproteins Encapsulated in Reversed Micelle  

作　　者：YANGYong MAOLu-yuan LILiu-zhu LIUXiao-guang SHIJun CAOShao-kui 

机构地区：[1]LabofBiomimeticMacromolecule,CollegeofMaterialsEngineering,ZhengzhouUniversity,Zhengzhou450052,P.R.China

出　　处：《Chemical Research in Chinese Universities》2004年第2期240-243,共4页高等学校化学研究（英文版）

基　　金：SupportedbytheNaturalScienceFundofHenanProvince(No.20024300006)andbytheNaturalScienceFundofHenanProvinceHighEducationofChina(No.0211021000)

摘　　要：Hemeproteins encapsulated in reversed micelle formulated with di-2-ethylhexyl sulfosuccinate(AOT) was found to catalyze the polymerization of o-phenylenediamine(o-PDA) with hydrogen peroxide, whereas o-PDA catalyzed by hemeproteins dissolved in water could only form its trimers. As the nanostructural environment in reversed micelle acts as a certain orientation surrounding medium, it offers a strong electrostatic field that alters the reductive potential of Fe 3+/Fe 2+(E m7) in the heme of hemeproteins and thus increases the catalytic activity of peroxidase accordingly. According to the results of UV-Vis, 1H NMR and FTIR, the polymer catalyzed by hemoglobin(Hb) in reversed micelle was presumed to be constructed of lines and trapeziforms alternatively.Hemeproteins encapsulated in reversed micelle formulated with di-2-ethylhexyl sulfosuccinate(AOT) was found to catalyze the polymerization of o-phenylenediamine(o-PDA) with hydrogen peroxide, whereas o-PDA catalyzed by hemeproteins dissolved in water could only form its trimers. As the nanostructural environment in reversed micelle acts as a certain orientation surrounding medium, it offers a strong electrostatic field that alters the reductive potential of Fe 3+/Fe 2+(E m7) in the heme of hemeproteins and thus increases the catalytic activity of peroxidase accordingly. According to the results of UV-Vis, 1H NMR and FTIR, the polymer catalyzed by hemoglobin(Hb) in reversed micelle was presumed to be constructed of lines and trapeziforms alternatively.

关 键 词：HEMEPROTEIN HEMOGLOBIN Horseradish peroxidase Reversed micell O-PHENYLENEDIAMINE 

分 类 号：TQ463[化学工程—制药化工]