丹参酮IIA对自发性高血压大鼠左室肥厚的影响及机制  

The effect of tanshinoneIIA preventing left ventricular hypertrophy in spontaneously hypertensive rats and its mechanism

作　　者：谢辉[1] 郑智[1] 龚丽娅[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,武汉市430030

出　　处：《实用医学杂志》2004年第3期252-254,共3页The Journal of Practical Medicine

基　　金：湖北省科技攻关课题 (2 0 0 2AA30 1C2 9)

摘　　要：目的 :观察丹参酮IIA对自发性高血压大鼠左室肥厚的作用 ,并探讨其作用机制。方法 :18只 8周龄的自发性高血压大鼠随机分成 3组 :一组于 8周处死 ,另两组分别经腹腔注射丹参酮IIA和蒸馏水 (1g·kg-1·d-1) ,共 10周。测量大鼠尾动脉收缩压 (SBP)及左心室重量指数 (LVMI)。应用HE染色、VG染色、免疫组织化学的方法 ,结合计算机图像分析技术 ,检测心肌细胞的直径和面积、心肌组织胶原体积比例 (CVF)、血管周围胶原面积和管腔面积比例 (PVCA)以及蛋白激酶C(PKC)的表达。结果 :与 8周龄的自发性高血压大鼠相比 ,18周龄大鼠的SBP、LVMI、心肌细胞的直径、面积、CVF、PVCA显著增加 ,PKC表达上调 ,丹参酮IIA治疗可抑制LVH的发展和心肌组织PKC的表达 ,但收缩压无明显改变。结论 :长期应用丹参酮IIA治疗可预防自发性高血压大鼠左室肥厚的形成 。Objective To observe the effect of tanshinoneIIA(TSN) on preventing left ventricular hypertrophy(LVH)in spontaneously hypertensive rats, and to investigate its mechanism. Methods 18 spontaneously hypertensive rats,8 weeks old,were divided into three groups at random. TSN and distilled water(1 g·kg -1 ·d -1 )were injected intraperitoneally into two groups, respectively for 10 weeks. Systolic blood pressure(SBP)and left ventricular mass index(LVMI)were measured.Combined with computed morphometry, HE, VG and immunohistochemical staining combined with computed morphometry were imployed to evaluate the cardiomyocyte size, diameter, the collagen volume fraction(CVF),perivascular circumferential area(PVCA),and protein kinase C(PKC)expression in the left ventricular tissue. Results Compared with 8 week old spontaneously hypertensive rats, the SBP,LVMI, cardiomyocyte size, diameter, CVF, PCVA, and PKC expression increased markedly in the 18 week old rats. Treated with TSN,the LVH was prevented and PKC expression decreased simultaneously whereas SBP changed slightly. Conclusion Chronic use of TSN can inhibit the development of LVH in spontaneously hypertensive rats .The mechanism may be that TSN decreases the expression of cardiac PKC.

关 键 词：丹参酮ⅡA 自发性高血压 大鼠 左室肥厚 作用机制 心肌细胞 蛋白激酶C 

分 类 号：R285[医药卫生—中药学]