FTY720对小鼠外周血淋巴细胞及T细胞亚群的影响  

Observation of influence of FTY720 on peripheral blood lymphocytes and T cell subsets of mice in vivo

作　　者：张雷[1] 左利群[2] 沈世乾[2] 朱彤[2] 朱有华[1] 闵志廉[1] 陈忠华 

机构地区：[1]第二军医大学长征医院泌尿外科,上海200002 [2]华中科技大学同济医院器官移植研究所

出　　处：《中华泌尿外科杂志》2004年第1期21-23,共3页Chinese Journal of Urology

基　　金：国家自然科学基金资助项目 (3 9970 70 5 )

摘　　要：目的　观察FTY72 0对近交系小鼠淋巴细胞及T细胞亚群变化的影响以指导临床用药。　方法　选用雄性C5 7/bl近交系小鼠。FTY72 0 3mg·kg-1·d-1,连续口服 14d。在各所选时间点计算淋巴细胞百分比 ,流式细胞术分析T细胞亚群变化。　结果　服用FTY72 0后 ,小鼠外周血淋巴细胞迅速下降 ,并于 4h降至最低值 ,之后保持稳定。停用FTY72 0后小鼠外周血淋巴细胞仍在低水平徘徊 2周 ,第 3周开始出现明显回升 ,停药后第 6周恢复至服药前水平。T细胞在服用FTY72 0后的变化与淋巴细胞相似。CD-8T细胞对FTY72 0相对较敏感。　结论　FTY72 0对外周血淋巴细胞的作用起效迅速 ,安全稳定 ,特异性强 ,可逆。对T细胞及其亚群的作用基本符合以上特点 ,CD-8T细胞对FTY72 0相对较敏感。Objective To investigate the influence of FTY720 on the lymphocytes and their recovery after withdrawal of FTY720,and on the changes in T cell subsets of inbred mice so as to direct the administration of FTY720. Methods Male inbred C57/bl mice were given FTY720 at the dose of 3 mg/kg per day by oral gavage once a day for 14 days. The PBLs were counted and T cell subsets were analyzed by flow cytometry at the selected time points. Results When FTY720 was administrated, the PBLs showed a sharp decrease and reached the minimum 4 hours later.Then the PBLs remained stable during the whole pe- riod of administration.The PBLs didn’t restore immediately when the reagent was withdrawn,but increased slowly 2 weeks later and completely recovered 6 weeks later.The changes of T cells were similar to PBLs.CD - 8 subsets showed relatively higher sensitivity to FTY720. Conclusions FTY720 is a safe reagent which has rapid and specific effects on PBLs,and the changes in PBLs are reversible.The effect of FTY720 on T cell subsets is similar to that on PBLs.CD - 8 T cells are relatively more sensitive to FTY720.

关 键 词：淋巴细胞 FTY720 T细胞亚群 外周血 流式细胞术 免疫抑制剂 合理用药 

分 类 号：R965[医药卫生—药理学]