仔猪腹泻抗性相关特异表达ESTs的筛选(英文)  

Screening Specifically Expressed ESTs Related to Piglet Diarrhea Resistance

作　　者：亓小红[1] 张勤[1] 刘培琼[2] 宋春云[1] 徐如海[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094 [2]贵州大学动物科学学院,贵阳550001

出　　处：《Acta Genetica Sinica》2004年第3期251-256,共6页

基　　金：国家重大基础研究发展规划(973计划)项目资助(编号:G2000016103)~~

摘　　要：以久仰香猪、剑白香猪和长白猪 3个品种的脾脏为材料 ,以品种间同一组织不同的发病状态为对照 ,将DDRT PCR技术与银染法相结合进行差异显示研究 ,筛选与仔猪腹泻抗性相关的特异表达ESTs。在脾脏未发病RNA池中检测到 5条特异表达ESTs ,其中一条与Nck转导蛋白同源 ,一条与长散布元件反转录酶同源 ,一条为相似性较高的未知功能序列 。RNA was extracted from spleens of diarrhea and non-diarrhea piglets of Jiuyang Pig,Jianbai Pig and Landrace.RNA pools were established and DDRT-PCR using three anchored and seven arbitrary primers combined with silver staining was conducted to identify ESTs differentially expressed in diarrhea resistance to E.coli K88.Five cDNA were identified in the non-diarrhea RNA pools.Among them two are new sequences and three are highly identical to the ESTs in the GeneBank,of which two have known functions.One is the mammalian Nck adaptor protein 1 and the other is the human LINE-1 reverse transcriptase.

关 键 词：仔猪腹泻 抗病性 DDRT—PCR EST 信号转导蛋白 长散布元件反转录酶 

分 类 号：S828[农业科学—畜牧学]