高效液相色谱法测定面刺霜中甲硝唑、氯霉素的含量  

Simultaneous Determination of Metronidazole and Chloramphenicol in Comedo Cream by HPLC

作　　者：罗东[1] 王章阳[1] 代青[1] 冉光炳[1] 彭蓓[2] 

机构地区：[1]第三军医大学西南医院药学部,重庆市400038 [2]重庆沙坪坝区人民医院药剂科,重庆市400036

出　　处：《中国药房》2004年第4期241-242,共2页China Pharmacy

摘　　要：目的:建立同时测定面刺霜中甲硝唑和氯霉素含量的方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(250mm ×4.6mm,5μm),流动相为甲醇(?)水(50:50),检测波长为280nm.结果:甲硝唑、氯霉素在10.0～250.0μg/ml浓度范围内均有良好的线性关系,相关系数均为0.9 998,平均回收率分别为100.54％、100.53％,相对标准差分别为0.79％,0.56％ 结论:本方法分离度好、快速、简便,可用于含甲硝唑、氯霉素霜剂的质量控制。OBJECTIVE: To establish a HPLC method for simultaneous determination of metronidazole and chloram. phcnicol in comedo cream. METHODS: The method included a Diamonsil C18 column (250mm × 4.6mm, 5μm) and methanol water(50 : 50,v/v)as the mobile phase and an ultraviolet detecting wavelength of 280nm .RESULTS:The calibralion curve was linear in a concentration range of ( 10 - 250)μg/ml of both metronidazole and chloramphenicol (r = 0.9 998) .The average recoveries of metronidazole and chloramphenicol were 100.53% , 100.53% and RSD were 0.79% , 0.56% respectively . CONCLUSION:The method for the determination of metronidazole and chloramphenicol was rapid and convenient. It can be used for the quality control of metronidazole and chloramphenicol in cream.

关 键 词：高效液相色谱法 甲硝唑 氯霉素 含量测定 

分 类 号：R927.2[医药卫生—药学]