人全血短期培养淋巴细胞程序外DNA合成的实验研究  

Study of Unscheduled DNA Synthesis Test in Short-term Cultured Human Whole Blood Lymphocytes

作　　者：韩成龙[1] 欧阳学[1] 李良寿[1] 海春旭[1] 蒋豫图[2] 

机构地区：[1]第四军医大学,西安710032 [2]军事医学科学院

出　　处：《中国公共卫生学报》1992年第6期358-360,共3页

基　　金：国家自然科学基金资助

摘　　要：通过将人全血细胞在体外3h 短期培养,同时用羟基脲(10mmol)抑制 S 期半保留 DNA 复制,以盐酸氮芥作用于细胞,用~3H-TdR 掺入,以液闪计数法,研究程序外 DNA 合成(UDS)。结果表明,全血短期培养淋巴细胞能显示较好的 UDS 反应,并与作用物盐酸氮芥的浓度有良好的剂量效应关系。对117例健康人 UDS 的测定表明,男女性 UDS 反应无显著性差别(P>0.05),但 UDS 反应随年龄增高而增加(P<0.01)。正常人与恶性肿瘤患者的 UDS 值,差别不显著。UDS test with short-term cultured HWBL were established.In this test,hydroxyurea(10 mM) was used to suppress half-preserved DNA duplication and,after exposing to DNA dama- ging agent for 3 hours,the incorporated ~3H- TdR in cell DNA was assayed by liquid scien- tillation counting set to reflect the extent of DNA damage and repairing ability of the cells, This test was easy and quick to perform and may be used in screening carcinogens and in cancer epidemiologic investigation. Lymphocyte UDS value of 117 normal per- sons (male 93,female,24) were assayed after exposing to NH_2·HCL (2.6×10^(-4)M).The mean UDS value was 1.95±0.67 and showed an incre- asing trend with age,indicating that the aged persons might be more susceptible to mutagens. UDS value showed no significant difference between males and females(1.98±0.66 vs.1.86 ±0.61).Comparing lymphocyte UDS value of 25 cancer patients with 74 normale controls,no significant difference on UDS was found betw- een Cancer cases and the controls (p>0.05).

关 键 词：淋巴细胞 培养 程序外 DNA合成 

分 类 号：R115[医药卫生—公共卫生与预防医学]