下丘脑β-EP及POMC mRNA的表达在针刺镇痛后效应中的作用  

Roles of Hypothalamic β-EP Level and POMC mRNA Expression in Acupuncture Analgesia

作　　者：陈瑾[1] 刘光谱[1] 周春阳[1] 

机构地区：[1]成都中医药大学,成都610075

出　　处：《针刺研究》2004年第1期5-9,共5页Acupuncture Research

基　　金：四川省教委课题

摘　　要：目的 :探讨下丘脑β EP及POMCmRNA的表达在针刺镇痛后效应中的作用。方法 :以急性佐剂性关节炎 (AA)大鼠作为炎症痛模型 ,针刺“昆仑”穴 ,观察即时及后效应 ;采用放免、原位杂交等方法检测下丘脑 β 内啡肽 ( β EP)含量及 β EP前体前阿黑皮素POMCmRNA的表达。结果 :针刺能提高AA大鼠痛阈并能持续 1hr以上 ,提高下丘脑 β EP含量并能持续 3 0min以上 ,促进下丘脑β EP前体POMCmRNA的表达并能持续 1hr以上。结论 :针刺镇痛有即时效应 ,也存在较为显著的后效应 ,下丘脑β EP及POMCmRNA的表达可能参与了这一过程。Objective:To observe the effect of changes of hypothalamic β EP level and expression of pro opiomelanocortin (POMC)mRNA on aftereffects of acupuncture analgesia.Methods:132 SD rats were evenly randomized into control group, model group and acupuncture group, and each group was then divided into 0 min, 15 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 12 hr, 18 hr and 24 hr subgroups, with 8 cases in each of the former 4 subgroups and 2 cases in each of the latter 6 subgroups. Rat inflammatory pain model (acute adjuvant arthritis) was made by injecting Freund's complete adjuvant (0.1 mL) into the right hindpaw. Right“Kunlun'(BL 60) was punctured with a filiform needle and retained for 30 min, when, the needle was manipulated once again every 5 min. Then, the pain threshold (latency of hindpaw withdrawal in responding to radiation heat stimulation) of the right hindpaw was determined with a radiation heat pain detector. Hypothalamus tissues of some rats of the abovementioned 10 subgroups were isolated to be cut into sections (6～8 μm) that were stained with in situ hybridization technique for displaying expression of POMC mRNA, and β EP content of hypothalamus tissue was determined with radioimmunoassay. Results:The pain threshold values of model group at 0 min, 15 min, 30 min and 1 hr were significantly lower than those of control group ( P < 0.01 ), and those of acupuncture group were all considerably higher than those of model group ( P < 0.01 ) despite being still markedly lower than those of control group at 0 min and 1 hr ( P< 0.05, 0.01 ). Comparison among the 3 groups showed that β EP contents of model group and acupuncture group at 0 min, 15 min, 30 min and 1 hr were significantly higher than those of control group ( P < 0.05,0.01 ); and those of acupuncture group were even markedly higher than those of model group at 0 min, 15 min and 1 hr ( P < 0.05,0.01 ). Changes of POMC mRNA expression in these 3 groups presented a similar tendency to those of β EP contents. Conclusion: Ac

关 键 词：下丘脑 Β-EP POMC mRNA 针刺镇痛 佐剂性关节炎 动物模型 内源性阿片肽 

分 类 号：R246.2[医药卫生—针灸推拿学]