针刺对吗啡戒断大鼠脑组织一氧化氮合酶基因表达的影响  

Effect of Electroacupuncture on Nitric Oxide Synthase mRNA Expression in Rats with Morphine Abstinence Syndrome

作　　者：宋小鸽[1] 唐照亮[1] 侯晓荣[1] 张荣军[1] 

机构地区：[1]安徽中医学院针灸经络研究所,合肥230038

出　　处：《针刺研究》2004年第1期39-42,共4页Acupuncture Research

基　　金：安徽省重点科研项目 (No.0 0 0 2 2 0 84 )

摘　　要：目的 :观察针刺对吗啡戒断大鼠脑组织神经元一氧化氮合酶基因 (nNOSmRNA)表达的影响 ,探讨针刺改善戒断症状的分子生物学机理。方法 :建立大鼠吗啡自然戒断模型 ,运用逆转录聚合酶链反应 (RT PCR)测定戒断大鼠脑组织nNOSmRNA的表达。观察戒断后 ,针刺“足三里”和一氧化氮合酶 (NOS)抑制剂L N 硝基精氨酸 (L NAME)对吗啡戒断大鼠戒断症状和脑组织nNOSmRNA表达的影响。结果 :戒断组nNOSmRNA表达增加 ,针刺组和L NAME组nNOSmRNA表达比戒断组减少。针刺组戒断积分比戒断组少 ,组间差异显著 (P <0 .0 1 )。结论 :针刺“足三里”穴具有抑制吗啡戒断大鼠脑组织nNOSmRNA表达的作用 ,提示这可能是针刺改善吗啡戒断症状的一个重要机制。Objective: To observe the effect of electroacupuncture (EA) of 'Zusanli'(ST 36) on nitric oxide synthase (NOS) mRNA expression in the brain in rats with morphine abstinence syndrome for studying the biomolecular mechanism of acupuncture in improving abstinence syndrome. Methods: Rat abstinence syndrome model was established by continuous muscular injection of morphine to the hindlimbs for 15 days. A total of 24 rats were randomly and evenly assigned to abstinence syndrome (AS) group, EA (ST 36) group and L nitroarginine (NAME) group. Expression of nNOS mRNA in the brain tissues was determined by using the reverse transcription polymerase chain reaction (RT PCR) method. EA (2/100 Hz, 10 V, duration of 30 min/time, once daily and 7 days altogether) was delivered to bilateral ST 36 via two inserted needles. Results: Comparison of abstinence symptoms among the three groups showed that the scores of EA and L NAME groups were significantly lower than those of AS group at 24 hr, 48 hr and 72 hr after withdrawal of morphine injection ( P <0.01). In comparison with AS group, nNOS mRNA expression of EA group and L NAME group was lower clearly, suggesting a suppression of nNOS mRNA expression by EA. Conclusion: In morphine natural abstinence syndrome rats, acupuncture of ST 36 may down regulate nNOS mRNA expression in brain tissues.

关 键 词：针灸疗法 吗啡戒断 大鼠 脑组织 一氧化氮合酶 基因表达 分子生物学 逆转录聚合酶链反应 

分 类 号：R277.796.1[医药卫生—中医学] R246.6