猫体外循环时心肌caspase3 mRNA表达的变化  

Expression of caspase 3 mRNA in feline myocardium during cardiopulmonary bypass

作　　者：曾志勇[1] 王志农[1] 何斌[1] 徐志云[1] 张宝仁[1] 

机构地区：[1]第二军医大学长海医院胸心外科,解放军胸心外科研究所上海200433

出　　处：《第二军医大学学报》2004年第4期383-385,共3页Academic Journal of Second Military Medical University

基　　金：上海市曙光计划资助项目 ( 0 2 SG3 0 ) ;上海市青年科技启明星计划资助项目 ( 0 0 QB14 0 5 3 )

摘　　要：目的 :观察猫体外循环 (CPB)时心肌 caspase3m RNA表达的变化。方法 :根据人和鼠 caspase3m RNA基因序列 ,利用 DNASIS软件设计猫 caspase3基因的 PCR引物 ,采用 RT- PCR检测 CPB过程中心肌 caspase3m RNA的表达。结果 :CPB主动脉阻断 (ACC)过程中猫心肌 caspase3m RNA的表达与 ACC前相比未见明显增高 ,再灌注 1 5 min时心肌 caspase3m RNA的表达量较 ACC前明显增加 (P<0 .0 1 ) ,再灌注 90 m in时心肌 caspase3m RNA的表达量为 ACC前的 4倍 ,差异有显著性意义 (P<0 .0 1 )。结论 :CPB中的缺血再灌注损伤可导致心肌 caspase 3基因表达增加 ,而再灌注过程可能是 caspaseObjective:To observe the expression of caspase 3 mRNA in feline myocardium during cardiopulmonary bypass (CPB). Methods:Primer of mutiple PCR detection for feline caspase 3 was designed according to the homology of the sequences of caspase 3 in humans,rats and mice registered in GenBank,and the expression of caspase 3 mRNA in myocardium during CPB was measured by the RT PCR method. Results:The caspase 3 mRNA expression in myocardium kept unchanged during period of aortic cross clamping (ACC),which was significantly increased at 15 min after reperfusion. At 90 min of reperfusion,caspase 3 mRNA expression in myocardium was 3 times higher than that before ACC ( P <0.01). Conclusion:Ischemia and reperfusion related over expression of caspase 3 in myocardium during CPB may be a reperfusion dependent process.

关 键 词：体外循环 心肌 caspase3mRNA 表达 基因序列 

分 类 号：R654.1[医药卫生—外科学]