AgNO_3溶液对兔肠去黏膜作用的实验研究  

De-epithelialization of intestine by AgNO_3 solutions in rabbits-an experimental study

作　　者：刘修恒[1] 程洪涛[1] 王淑慧[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《肿瘤防治研究》2004年第4期220-222,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　观察不同浓度的AgNO3 溶液在不同时间内对兔肠的去黏膜作用 ,探讨其对肠膀胱成形术的临床意义。方法　将 4 0只大白兔随机分 4组 ,每组 10只 ,手术游离 15cm空肠 ,保留该肠段血供 ,肠吻合恢复肠的连续性。除对照组 (A组 )外 ,对其余三组 (B、C、D组 )游离肠段分别给于 2 %AgNO3 溶液 30min 5 %AgNO3 溶液 10min、手术剥除肠黏膜处理 ,分别测量各组游离肠段在内压为 14cm/H2 O时的容量。所有兔子 (存活 33只 )于第 8周末处死取游离空肠 ,测定其容量 ,进行病理组织学检查及分析。结果　B组可以部分去除肠黏膜 ,C、D组可以完全去除肠黏膜。仅D组有显著胶原纤维形成及肠管容量减少。结论　AgNO3 溶液有去肠黏膜作用 ,5 %AgNO3 溶液 10min处理肠段可以完全去除肠黏膜 ,无显著纤维化 ,对肠膀胱成形术具有重要的临床意义。Objective To study the de-epithelialization effect of intestine by different concentrations of AgNO_3 solutions at different time points in rabbits and approach its clinical significance on enterocystoplasy.Methods Forty rabbits were randomly divided into four groups (10 rabbits each group). All the rabbits were anesthetized before operation. For each rabbit,one 15cm segment of jejunum was isolated with intact mesentety. Intestinal anastomosis was performed to keep its continuity. The segments from group B were treated with 2g/100ml silver nitrate (AgNO_3) solution for 30 minutes, the segments from group C were treated with 5g/100ml AgNO_3 solution for 10 minutes,mucosa on the segments from group D were stripped by surgical blade. The control group (Group A) is not treated with any measure. Volume of free intestine was measured when the inner pressure was 14cm/H_2O. Thirty-three rabbits survived the operation and were killed 8 weeks after operation for histopathological analysis.Results Histopathological analysis demonstrated that almost complete de-epithelialization of the segments from group C and group D,which did not occur in the segments from other groups. Severe retraction and fibrosis were observed on the segments from group D,but not on the other groups.Conclusion AgNO_3 solution can effectively inactivate intestinal mucous. Ten minutes' treatment of 5% AgNO_3 solution can successfully de-epithelialize jijunal segments without creating severe retraction or fibrosis. This novel treatment can be very useful for enterocystoplasy.

关 键 词：肠膀胱成形术 AgNO3溶液 肠黏膜 组织形态学 胶原纤维 杀菌剂 

分 类 号：R979.7[医药卫生—药品] R574[医药卫生—药学]