雌激素对去卵巢大鼠记忆功能、脑海马部位Aβ1-40及PKC表达的影响  

The effects of estradiol on memory function and the expression of amyloid-beta protein 1-40 and protein kinase C immunoreactivity in hippocampus of ovariectomized rats

作　　者：寇宗莉[1] 李茂欣[1] 汤旭磊[1] 高林[1] 吕海红[1] 

机构地区：[1]兰州医学院第一附属医院内分泌研究室,甘肃兰州730000

出　　处：《中国老年学杂志》2004年第4期350-352,共3页Chinese Journal of Gerontology

摘　　要：目的　探讨雌激素对去卵巢大鼠记忆功能、海马部位 β淀粉样蛋白 1 - 4 0 (Aβ1 - 4 0 )及蛋白激酶 C(PKC)表达的影响。方法　大鼠去卵巢建立动物模型 ,1 7w行水迷宫试验 ,1 8w取脑组织做 Aβ1 - 4 0及 PKC的免疫组化染色。结果　去卵巢大鼠的雌激素 (E2 )水平明显降低 (P<0 .0 1 ) ;去卵巢组的记忆功能下降 ;脑海马部位的 Aβ1 - 4 0表达比假手术组和雌激素替代组增加 (P<0 .0 1 ) ,而 PKC表达则减少 (P<0 .0 1 )。结论　雌激素可能是通过提高 PKC的表达水平 ,使淀粉样前体的代谢生成 Aβ1 - 4 0减少来保护海马部位胆碱能神经元 ,从而改善记忆功能。雌激素可能是影响记忆功能重要因素之一。Objective To investigate the effects of estradiol on memory function and the expression of amyloid-beta protein 1-40 (Aβ1-40) and protein kinase C (PKC) in the hippocampus of ovariectomized mice. Methods Using the animal model of ovariectomized mice, we detected the memory function of rats by morris water maze in the 17th week and got the brain to do immunohistochemical staining of Aβ1-40 and PKC in the 18 th week. Results The level of estradiol was lower (p<0.01) and the memory function was weaker in the ovariectomized group significantly. Aβ1-40 immunoreactivity positive neurons distributed abundantly but PKC immunoreactivity positive neurons reduced in hippocampus in the ovariectomized mice compared with the other two groups. Conclusions Estradiol can improve the memory function maybe by increasing the expression of PKC to reduce the production of Aβ1-40 protein. Estradiol may be one of the important factors for memory function.

关 键 词：雌激素 去卵巢大鼠 记忆功能 脑海马部位 AΒ1-40 PKC Β淀粉样蛋白1-40 蛋白激酶C 

分 类 号：R338.64[医药卫生—人体生理学] Q579.13[医药卫生—基础医学]