慢性乙型肝炎患者外周血CD14^+细胞的功能状态  

CD14^+ cell dysfunction in patients with chronic active hepatitis B

作　　者：魏然[1] 夏作理[1] 陈彬[2] 韩纪举[2] 任道凌[2] 杨明峰[2] 

机构地区：[1]山东大学医学院生理教研室,山东省济南市250012 [2]泰山医学院基础医学研究所,山东省泰安市271000

出　　处：《世界华人消化杂志》2004年第3期618-621,共4页World Chinese Journal of Digestology

摘　　要：目的:研究慢性乙型肝炎患者外周血CD14+细胞早期活化抗原分子表达、炎症因子生成和吞噬能力. 方法:采用四色荧光分析方法,运用流式细胞术检测正常对照人群(10例)、慢性活动性乙型肝炎患者(11例)外周血单个核细胞(PBMC)CD14+细胞率和CD14抗原量;并行CD14设门,检测CD14+细胞前向角(FSC)、侧向角(SSC) 值、细胞早期活化抗原(CD69)的表达率、TNF-α表达率、细胞内TNF-α水平、细胞吞噬率及吞噬力. 结果:HB组CD14+细胞SSC值高于对照组(P<0.01);HB 组和对照组间外周血CD14+细胞比例、CD14抗原水平无统计学意义差别,抗原水平与CD14+细胞率呈正相关;HB 组CD14/CD69双表达细胞明显多于对照组(P<0.01);HB组CD14/TNF-α+(细胞内)双染细胞与对照组间无差别,HB 组CD14+细胞TNF-α水平高于正常对照组(P<0.05);对照组TNF-α细胞率与细胞颗粒度呈正相关,与CD14/69双表达并发生吞噬细胞率一致.HB组CD69/CD14双阳性细胞荧光球吞噬率与对照组无差别(P>0.05):HBV组CD14+ 细胞荧光球吞噬率显著高于对照组(P<0.01),吞噬力低于对照组(P<0.05),荧光微球吞噬率与CD14抗原表达的量负相关;CD14+细胞SSC,TNF-α细胞率同CD14抗原表达的量也呈负相关. 结论:慢性乙肝患者外周血存在过度活化CD14+单核细胞,细胞吞噬力弱、胞内TNF-α生成增多.AIM: To study the functional states of CD14+ cells in peripheral blood mononuclear cell (PBMCs) by analyzing the expression of early active antigen, production of pro-inflammatory cytokines and phagocytosis with flow cytometry (FCM). METHODS: PBMCs were isolated from 11 patients with chronic active hepatitis B and 10 healthy blood donors, and 4-color flow cytometry and gate setting techniques were applied. The percentage of CD14+ cells and level of CD14 antigen were tested by FCM, after gate setting with CD14, side scatter (SSC) and activation-linked surface antigen (CD69) expression rate, percentage of TNF-αproducing cells, level of intro-cellular TNF-α, the percentage of phagocytes and the ability of phagocytosis were investigated. RESULTS: The SSC mean value of CD14+ cell of HBV patients was higher than that of health control (P<0.01); There were no difference between two testing groups on the percentage of CD14+ cells and level of CD14 antigen, however the level of CD14 antigen was positively associated with the percentage of CD14+ cells. CD14/69 double expression rate was significantly higher than that of control group (P <0.01); No difference was observed on CD14/ TNF-α double stain cells between testing groups; In comparison with control group, the level of cellular TNF-α in HBV group was higher (P <0.05). In control group, the percentage of TNF-α producing cells were correlated with SSC value and number of phagocytes that expressed CD14/ 69. There was no difference in phagocytes that expressed CD14/69 in two groups. As compared with the control group, there was more phagocytostic CD14+ cells in HBV group (P <0.01), but the mean phagocytosis amount was significantly lower in this group (P<0.05), and the number of phagocyte was negatively correlated with the level of CD14 antigen; while the SSC value and number of TNF-α producing cells were negatively correlated with level of CD 14 antigen. CONCLUSION: CD14+ cells in PBMCs from chronic active hepatitis B patients are over activated in a dysfunction st

关 键 词：慢性乙型肝炎 外周血 CD14^+细胞 炎症因子 流式细胞术 炎症反应 

分 类 号：R512.62[医药卫生—内科学]