大鼠短间隔连续部分肝切除中再生肝的基因表达差异  

Gene expression diflerence in regenerating rat liver after 0-36-40-44h short interval successive partial hepatectomy

作　　者：徐存拴 韩鸿鹏[1] 袁金云[1] 常翠芳[1] 李文强 杨柯金[1] 赵利峰[1] 李玉昌 张会勇 Salman Rahman 章静波 

机构地区：[1]河南师范大学生命科学学院,河南省新乡市453002 [2]细胞分化调控国家重点实验室培育基地,河南省新乡市453002 [3]伦敦大学血友病研究中心

出　　处：《世界华人消化杂志》2004年第3期654-663,共10页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.30270673国家重点实验室基金资助项目;No.030001~~

摘　　要：目的:鉴定0-36-40-44 h大鼠短间隔连续部分肝切除(0- 36-40-44 h SISPH)中与肝再生有关的基因,并分析他们在肝再生中的表达动态和作用. 方法:从0-36-40-44h SISPH再生肝消减cDNA文库中筛选与肝再生有关的基因,用基因芯片技术分析其在肝再生中的表达动态,并用GeneMath软件对有关基因进行聚类分析. 结果:用基因芯片技术分析的551个与肝再生有关基因中, 157个基因至少在肝再生的—个时间点表达变化达2倍以上,其中,上调表达的基因有87个,下调表达的基因有70个;157个基因中的71个属未报道的基因,86个为已报道基因,但在此之前尚不清楚他们与肝再生有关;聚类分析和统计学分析表明,0-36-40-44 h SISPH的基因表达模式分为6类,即早期诱导,中期诱导,晚期诱导,早期抑制,中期抑制,晚期抑制.与PH相比,38个基因在0-36-40-44 h SISPH中特异性表达,119个基因在这两个模型中表达趋势相同,但在各时间点的表达丰度不同. 结论:0-36-40-44 h SISPH中,上调和下调表达的基因数目相差不大;晚期表达的基因多于早期表达的基因,远多于中期表达的基因;表达幅度小的基因(2-5倍)多于表达幅度大的基因.AIM: To identify genes related to rat liver regeneration (LR) after 0-36-40-44h short interval successive partial hepatectomy (SISPH) and to analyze their action and expression profile in LR. METHODS: A cDNA microarray containing 551 elements (liver chip) was made to analyze extensively expression changes of them in 0-36-40-44h SISPH, which were selected from subtractive cDNA libraries of the LR. Cluster analysis of these gene expression profile was performed by Genemath. RESULTS: Among the selected 551 cDNA, 157 were up- or down-regulated more than twofold at one or more time points. Of the 157 elements, 86 were up-regulated and 71 down-regulated, and 70 were not reported and 87 were reported, which had not been previously reported to be involved in LR. By cluster analysis and generalization analysis, 6 distinct temporal induction or suppression patterns snowed that immediate induction, intermediate induction, late induction, immediate suppression, intermediate suppression, and late suppression. Comparison of the gene expression in SISPH with after PH found that 38 genes were specially altered in SISPH, and the expression trends for other 119 genes were similar between SISPH and PH, except of the various abundance at the different time points. CONCLUSION: In 0-36-40-44h SISPH, the numbers of the up-regulated and down-regulated genes show no apparent difference. The genes expressed lately are more than that immediately, and much more than that intermediately. The genes expressed abundantly are much less than that increased 2-5 folds.

关 键 词：肝再生 cDNA文库 基因芯片技术 肝细胞 动物实验 细胞周期蛋白 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学] R394.8[医药卫生—临床医学]