HPLC法测定保胃丸中芍药苷的含量  

Determination of Paeoniflorin in Baowei Pill by HPLC FENG

作　　者：冯伟红[1] 李曼玲[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中医药信息杂志》2004年第3期222-223,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的 建立高效液相色谱法测定保胃丸中芍药苷的含量。方法 采用ODS色谱柱(4.6mm×250mm),以甲醇-异丙醇-36%乙酸-水(25∶2∶2∶71)为流动相;检测波长为230nm。结果 芍药苷的含量线性范围为0.4～2.0μg,r=0.9997,平均回收率为97.86%,RSD为1.99%。结论 该方法简便、准确。Objective To develop a HPLC method for determining Paeoniflorin in Baowei Pill. Method ODS column(4.6mm×250mm) was used with methanol-iso Propanol-36% acetic acid-water (25∶2∶2∶71) as mobile phase. Detection wavelength was 230 nm. Result Paeoniflorin was separated well. Good linearity of Paeoniflorin was obtained (r=0.9997) within the range of 0.4～2.0μg. The average recovery was 97.86% and RSD was 1.99%. Conclusion This method was simple and accuracy.

关 键 词：HPLC法 保胃丸 芍药苷 含量测定 色谱条件 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]