杂种杨无性系的光系统Ⅱ放氧活性、光合色素及叶绿体超微结构对光胁迫的响应  

RESPONSE OF OXYGEN EVOLUTION ACTIVITY OF PHOTOSYSTEM II, PHOTOSYNTHETIC PIGMENTS AND CHLOROPLAST ULTRASTRUCTURE OF HYBRID POPLAR CLONES TO LIGHT STRESS

作　　者：张守仁[1] 高荣孚[2] 王连军 

机构地区：[1]中国科学院植物研究所植被数量生态学重点实验室,北京100093 [2]北京林业大学,北京100083 [3]内蒙古兴安盟林业局,乌兰浩特137400

出　　处：《植物生态学报》2004年第2期143-149,共7页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金资助(3 0 2 70 2 3 7)

摘　　要：用氧电极仪、红外CO2 气体分析仪及叶绿素荧光仪,结合透射电镜技术对几个杂种杨无性系在光胁迫下的光系统Ⅱ活性、光合色素及叶绿体超微结构进行了测定。随着预处理光强的增加,各无性系叶片的净光合速率和光系统Ⅱ放氧活性明显下降。二倍体无性系B1 1的光系统Ⅱ最大光化学效率(Fv/Fm)及实际光化学效率[(Fm′ -F)/Fm′]高于两个三倍体无性系B346和B342。强光下三倍体无性系B346的非光化学淬灭远高于B342和二倍体无性系B1 1。叶绿素含量和光合速率没有明显的线性关系,叶绿素a/b含量在自然光胁迫下存在季节变化,受强光胁迫 2个三倍体无性系的叶绿素a/b增大。预处理光强PFD超过 30 0 0 μmolphotons·m-2 ·s-1 ,各无性系的基粒类囊体片层结构遭到破坏,但二倍体无性系的类囊体片层结构受破坏程度较三倍体无性系轻。叶绿体蛋白合成抑制剂(硫酸链霉素,SM)The photosynthetic rate, oxygen evolution activity of the PSⅡ center, and chloroplast ultrastructure of three hybrid poplar clones under light stress were investigated using an oxygen electrode, IRGA (infra_red CO 2 gas analyzer), chlorophyll fluorometer and transmission electron microscopy. The photosynthetic rate and PSⅡ oxygen evolution varied negatively with the pretreatment_PFD (Photon Flux Density), whereas the chlorophyll a/b varied positively with the pretreatment_PFD for two triploid clones. Both the maximal and effective PSⅡ efficiencies of the diploid clone B11 were much higher than those of the two triploid clones B346 and B342. The non_photochemical quenching of triploid B346 was higher than in the B342 and B11 clones. The photosynthetic capacity and resistance to light stress for the diploid clone was higher than in triploid clones. There was no close relationship between chlorophyll content and photosynthetic rate, there was seasonal variation in CHL content and CHLa/b, and the ratio of CHLa to CHLb decreased with enhancing pretreatment_PFDs. The chloroplast protein inhibitor streptomycin (SM) blocked the re_synthesis process of D1 protein. Transmission electron micrographs showed that 1h strong irradiance exposure could result in the decomposition of the lamellar structure of grana. Clones B11 and B342 were more resistant to light stress than clone B346.

关 键 词：光胁迫 杂种 杨 无性系 光系统Ⅱ放氧活性 叶绿素荧光 光合色素 叶绿体 超微结构 

分 类 号：S792.11[农业科学—林木遗传育种]