M-CSF TARGETING INTO LCL NUCLEUS BEHAVES AS A MALIGNANCY PROMOTOR  

作　　者：曹震宇 吴克复 宋玉华 李戈 林永敏 饶青 马小彤 

机构地区：[1]State Key Laboratory for Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020

出　　处：《Chinese Journal of Cancer Research》2003年第4期262-268,共7页中国癌症研究（英文版）

基　　金：This work was supported by the Climbing Program Granted by the Ministry of Science and Technology of China(95-special-10) and Tianjin Science and TechnologyDevelopment Project (No. 003119311).

摘　　要：Objective: To investigate the functions of nM-CSF in malignant cells. Methods: recombinant M-CSF was targeted into cell nucleus by employing a eukaryotic expression plasmid vector pCMV/myc/nuc. The constructed plasmid was transfected into cells of EBV transformed lymphoblastoid cell line (LCL). RT-PCR, Western blot and immunofluorescent staining showed that recombinant M-CSF was localized into LCL cell nucleus. The transgenic cells showed elevated proliferation potential, enhanced resistance to apoptosis and increased ability of in vitro migration. Conclusion: Nucleus presenting M-CSF might act as a promoting factor in the processes of cell malignancy.Objective: To investigate the functions of nM-CSF in malignant cells. Methods: recombinant M-CSF was targeted into cell nucleus by employing a eukaryotic expression plasmid vector pCMV/myc/nuc. The constructed plasmid was transfected into cells of EBV transformed lymphoblastoid cell line (LCL). RT-PCR, Western blot and immunofluorescent staining showed that recombinant M-CSF was localized into LCL cell nucleus. The transgenic cells showed elevated proliferation potential, enhanced resistance to apoptosis and increased ability of in vitro migration. Conclusion: Nucleus presenting M-CSF might act as a promoting factor in the processes of cell malignancy.

关 键 词：Macrophage colony-stimulating factor Nuclear localization sequence Lymphoblastoid cell line (LCL) Matrix metalloproteinase-2 APOPTOSIS 

分 类 号：R33[医药卫生—人体生理学]